Skip to main content
SpringerLink
Log in

Characterization by different techniques of adrenocorticotropin and gonadotropin producing cells in lerot pituitary (Eliomys quercinus)

A superimposition technique and an immunocytochemical technique

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

Adrenocorticotropin and gonadotropin producing cells were localized in the adenohypophysis of normal Lerots by using anti-β 1–24 ACTH, anti-LH, anti-LH β, anti PMSG antisera.

In order to study their fine structure two techniques were employed: a superimposition technique which consists of detailed comparisons between the same cells in light, fluorescence and electron microscopic preparations and an immunocytochemical technique on ultra-thin sections using the peroxidase anti-peroxidase complex technique.

The superimposition technique allows an excellent description of cell ultrastructure of individually identified cells of each type. With this method we were able to desdribe the corticotropin secreting cells as lucent cells with electron dense granules ranging in size from 2500 to 3500 Å.

The gondotropin secreting cells are darker and their granules are about 2000 Å in diameter.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barnes, B. G.: Electron microscope studies on the secretory cytology of the mouse anterior pituitary. Endocrinology 71, 618–628 (1962)

    Google Scholar 

  • Barnes, B. G.: The fine structure of the mouse adenohypophysis in various physiological states. In: Cytologie de l'Adénohypophyse (J. Benoit and C. Da Lage, ed.). Paris: Editions du Centre National de la Recherche Scientifique 1963

    Google Scholar 

  • Beauvillain, J.-C., Tramu, G.: Cellules à l'activité corticotrope de l'hypophyse du Lerot (Eliomys quercinus): superposition des résultats de microscopie optique (immunofluorescence et colorations) et de microscopie électronique. C.R. Acad. Sci. (Paris) 277, 1025–1028 (1973)

    Google Scholar 

  • Beauvillain, J.-C., Tramu, G., Dubois, M.P.: Individualisation ultrastructurale des cellules gonadotropes et corticotropes de Pantéhypophyse de Lérot et de Cobaye par superpositions de microscopie optique (immunofluorescence et colorations) et de microscopie électronique. Colloque Annuel de la S.F.M.E., 1974 Rennes, J. de Microscopie 20, 20a (1974)

  • Bugnon, C., Lenys, D., Herlant, M., Dessy, C.: Caractérisation de diverses cellules de l'adénohypophyse du Renard par immunofluorescence sur coupes semi-fines et superpositions des données de microscopie électronique. C.R. Acad. Sci. (Paris) 278, 1243–1248 (1974)

    Google Scholar 

  • Dessy, C., Herlant, M.: Localisation comparée des immunsérums antilipotropine, anticorticotropine, et antimélanotropine β au niveau de l'hypophyse du Porc. C.R. Acad. Sci. (Paris) 278, 1923–1926 (1974)

    Google Scholar 

  • Doerr-Schott, J., Dubois, M. P.: Mise en évidence des hormones de l'hypophyse d'un amphibien par la cyto-immuno-enzymologie au microscope électronique. C.R. Acad. Sci. (Paris) 276, 2179–2182 (1973)

    Google Scholar 

  • Dubois, M. P.: Cytologie de l'hypophyse des bovins: séparation des cellules somatotropes et des cellules à prolactine par immunofluorescence. Identification des cellules LH dans la pars tuberalis et la pars intermedia. Bull. Ass. Anat. (Nancy) 145, 139–146 (1969)

    Google Scholar 

  • Dubois, M. P.: Cytologie et immunocytologie de l'hypophyse de bovins. In: Fonction gonadotrope et rapports hypothalamo-hypophysaire chez les animaux sauvages (ed. by M. Herlant), p. 225–242. Paris: Masson & Cie 1971a

    Google Scholar 

  • Dubois, M. P.: Les cellules à hormones glycoprotidiques du lobe antérieur de l'hypophyse: séparation par immunofluorescence des cellules thyréotropes et des cellules gonadotropes dans l'hypophyse des bovins, ovins, porcins. Ann. Recher. Veter. 2, 197–222 (1971b)

    Google Scholar 

  • Dubois, M. P.: Les cellules corticotropes de l'hypophyse des bovins, moutons et porcs. Ann. Biol. Biochem. Biophys. 11, 589–624 (1971c)

    Google Scholar 

  • Dubois, M. P.: Localisation cytologique par immunofluorescence des sécrétions corticotropes, α et β mélanotropes au niveau de l'antéhypophyse des bovins, ovins et porcins. Z. Zellforsch. 125, 200–209 (1972a)

    Google Scholar 

  • Dubois, M. P.: Localisation par immunocytologie des hormones glycoprotidiques hypophysaires chez les vertébrés. In: Pituitary glycoprotein hormones, p. 27–47. Paris: I.N.S.E.R.M. 1972b

    Google Scholar 

  • Dubois, M. P.: Nouvelles données sur la localisation au niveau de l'adénohypophyse des hormones polypeptidiques, ACTH, MSH, LPH. Lille med. 17, 1391–1420 (1972c)

    Google Scholar 

  • Dubois, M. P.: Recherche par immunofluorescence des cellules adénohypophysaires élaborant les hormones polypeptidiques ACTH, α MSH, β MSH, Bull. Ass. Anat. (Nancy) 57, 63–76 (1973)

    Google Scholar 

  • Dubois, M.P., Graf, L.: Démonstration by immunofluorescence of the lipotropic hormone (LPH) in bovine, ovine and porcine adenohypophysis. Horm. metab. Res. 5, 229 (1973)

    Google Scholar 

  • Dubois, P., Dubois, M. P.: Mise en évidence par immunofluorescence de l'activité gonadotrope LH dans l'antéhypophyse foetale humaine. Colloque I.N.S.E.R.M., Sexual Endocrinology of the Perinatal Period, Lyon (France) p. 30–31 (1974) (sous presse)

  • Dubois, P., Vargues-Regairaz, M., Dubois, M. P.: Human foetal antehypophysis: immunofluorescent evidence for corticotropin and melanotropin activities. Z. Zellforsch. 145, 131–143 (1973)

    Google Scholar 

  • Farquhar, M. G., Rinehart, J. F.: Electron microscopic studies of the anterior pituitary gland of castrate rats. Endocrinology 54, 516–541 (1954)

    Google Scholar 

  • Girod, C.: Recherches sur les cellules gonadotropes antéhypophysaires du Hérisson: étude cytologique, ultrastructurale et cytophysiologique In: Fonction gonadotrope et rapport hypothalamo-hypophysaire chez les animaux sauvages (ed. by M. Herlant), p. 57–81. Paris: Masson & Cie 1971

    Google Scholar 

  • Herlant, M.: Ultrastructure des cellules gonadotropes de l'hypophyse chez les Mammifères. In: Hormones glycoprotéiques hypophysaires (M. Jutisz, ed.), p. 5–25. Paris: Editions I.N.S.E.R.M. 1972

    Google Scholar 

  • Herlant, M., Ectors, F.: Les cellules gonadotropes de l'hypophyse chez le porc. Z. Zellforsch. 101, 212–231 (1969)

    Google Scholar 

  • Herlant, M., Klastersky, J.: Etude au microscope électronique des cellules corticotropes de l'hypophyse. C.R. Acad. Sci. (Paris) 256, 2709–2712 (1963)

    Google Scholar 

  • Kurosumi, K., Kobayashi, Y.: Corticotrophs in the anterior pituitary glands of normal and adrenalectomized rats as revealed by electron microscopy. Endocrinology 78, 745–758 (1966)

    Google Scholar 

  • Kurosumi, K., Oota, Y.: Electron microscopy of two types of gonadotrophs in the anterior pituitary gland of persistent estrous and persistent diestrous rats. Z. Zellforsch. 85, 34–46 (1968)

    Google Scholar 

  • Leleux, P., Robyn, C.: Immunohistochemistry of individual adenohypophyseal cells. Acta endocr. (Kbh.) (Suppl.) 153, 168–189 (1971)

    Google Scholar 

  • Mayor, H. D., Hampton, J. C., Rosario, B.: A simple method for removing the resin from epoxy embedded tissue. J. biophys. biochem. Cytol. 9, 909–910 (1961)

    Google Scholar 

  • Mazurkiewicz, J. E., Nakane, P. K.: Immunocytochemistry on peg embedded tissues. J. Histochem. Cytochem. 20, 969–974 (1972)

    Google Scholar 

  • Mikami, S.: Light and electron microscopic investigations of six types of glandular cells of the bovine adenohypophysis. Z. Zellforsch. 105, 457–482 (1970)

    Google Scholar 

  • Moriarty, G. C., Halmi, N. S.: Electron microscopic study of the adrenocorticotropin producing cell with the use of unlabeled antibody and the soluble peroxidase antiperoxidase complex. J. Histochem. Cytochem. 20, 590–604 (1972)

    Google Scholar 

  • Naik, D. V.: Electron microscopic immunocytochemical localization of adrenocorticotropin and MSH in the pars intermedia cells of rats and mice. Z. Zellforsch. 142, 305–328 (1973)

    Google Scholar 

  • Nakane, P. K.: Classifications of anterior pituitary cell types with immunoenzyme histochemistry. J. Histochem. Cytochem. 18, 9–20 (1970)

    Google Scholar 

  • Pelletier, G.: Identification en microscopie électronique des cellules corticotropes chez le Rat: effet de la surrénalectomie associée ou non à un traitement par dexaméthasone. C.R. Acad. Sci. (Paris) 270, 2836–2840 (1970)

    Google Scholar 

  • Pelletier, G., Racadot, J.: Identification des cellules hypophysaires sécrétant l'ACTH chez le Rat. Z. Zellforsch. 116, 228–239 (1971)

    Google Scholar 

  • Phifer, R. F., Midgley, A. R., Spicer, S.S.: Immunohistologic and histologic evidence that follicle stimulating hormone and luteinizing hormone are present in the same cell type in the human pars distalis. J. clin. Endocr. 36, 125–141 (1973)

    Google Scholar 

  • Robyn, C., Leleux, P., Vanhaelst, L., Golstein, J., Herlant, M., Pasteels, J. L.: Immunohistochemical study of the human pituitary with anti LH and anti FSH and anti thyrotrophin sera. Acta endocr. (Kbh.) 72, 625–643 (1973)

    Google Scholar 

  • Saint Guillain, M., Dessy, C., Massant, B., Herlant, M.: Identification en microscopie électronique des cellules corticotropes chez le porcelet. C.R. Acad. Sci. (Paris) 278, 2185–2188 (1974)

    Google Scholar 

  • Siperstein, E. R., Miller, K. J.: Further cytophysiologic evidence for the cells that produce adrenocorticotrophic hormone. Endocrinology 86, 451–486 (1970)

    Google Scholar 

  • Siperstein, E. R., Miller, K. J.: Hypertrophy of the ACTH-producing cell following adrenalectomy: A quantitative electron microscopic study. Endocrinology 93, 1257–1269 (1973)

    Google Scholar 

  • Stefan, Y., Dubois, M.P.: Localisation par immunofluorescence des hormones corticotropes et mélanotropes dans l'hypophyse du rongeur Ellobius lutescens. Z. Zellforsch. 133, 353–365 (1972)

    Google Scholar 

  • Stefanini, M., De Martino, C., Zamboni, L.: Fixation of ejaculated spermatozoa for electron microscopy. Nature (Lond.) 216, 173 (1967)

    Google Scholar 

  • Sternberger, L. A., Hardy, P. H. J., Cuculis, J. J., Meyer, H. G.: The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigenantibody complex (horseradish peroxidase-antiperoxidase) and its use in identification of Spirochetes. J. Histochem. Cytochem. 18, 315–333 (1970)

    Google Scholar 

  • Tougard, C., Kerdelhue, B., Tixier-Vidal, A., Jutisz, M.: Light and electron microscope localization of binding sites of antibodies against ovine luteinizing hormone and its two subunits in rat adenohypophysis using peroxidase-labeled antibody technique. J. Cell Biol. 58, 503–521 (1973)

    Google Scholar 

  • Tramu, G., Beauvillain, J.-C., Dubois, M.P.: Cytologie adénohypophysaire du Lerot à diverses périodes du cycle annuel. Etude en immunof luorescence et microscopie électronique. Colloque Annuel de Neuroendocrinologie, 1974 Chizé. J. Physiol. (Paris) 68, 24 B (1974)

  • Tramu, G., Beauvillain, J.-C., Dubois, M. P.: Les cellules gonadotropes du Lerot: concordances des résultats de microscopie optique et de microscopie électronique. J. de Microscopie: submitted for publication (letters to the editor)

  • Tramu, G., Dubois, M. P.: Identification par immunofluorescence des cellules à activité LH du cobaye mâle. C.R. Acad. Sci. (Paris) 275, 1159–1161 (1972)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Histology of the Faculty of Medicine, Lille Cedex, France

    J.-C. Beauvillain, G. Tramu & M. P. Dubois

  2. Laboratory of the Physiology of Reproduction, INRA, CNRZ, Nouzilly, France

    J.-C. Beauvillain, G. Tramu & M. P. Dubois

Authors
  1. J.-C. Beauvillain
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Tramu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. P. Dubois
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

We thank Dominique Quief for his technical assistance. This work was supported by a grant from U.E.R. III Lille 1974.

Attachés de Recherche INSERM.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Beauvillain, JC., Tramu, G. & Dubois, M.P. Characterization by different techniques of adrenocorticotropin and gonadotropin producing cells in lerot pituitary (Eliomys quercinus). Cell Tissue Res. 158, 301–317 (1975). https://doi.org/10.1007/BF00223828

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00223828

Key words

Search

Navigation