Summary
The presence and distribution of galanin-immunoreactivity was examined in the uterine cervix and paracervical autonomic ganglia of the female rat. Some animals were treated with capsaicin to determine if galanin-immunoreactivity was present in small-diameter primary afferent nerves. Other animals were treated with the noradrenergic neurotoxin 6-hydroxydopamine to ascertain if galanin-immunoreactivity was present in sympathetic noradrenergic nerves. Galanin-immunoreactive nerve fibers were sparse in the cervical myometrium and vasculature, but numerous in the paracervical ganglion where they appeared to innervate principal neurons. Immunoreactivity was also present in dorsal root ganglia, dorsal horn of spinal cord, and inferior mesenteric ganglia. Capsaicin treatment resulted in a marked reduction of galanin-immunoreactivity in the spinal cord dorsal horn, but not in the dorsal root ganglia, paracervical ganglia, or cervix (although there was a substantial reduction of substance P-, neurokinin A-, and calcitonin gene-related peptide-immunoreactivity in the dorsal horn, dorsal root ganglia, and uterine cervix). 6-Hydroxydopamine treatment did not cause any appreciable change in the galanin-immunoreactivity in any tissues. We conclude that galanin-like immunoreactivity is expressed in nerve fibers innervating the paracervical ganglia and uterine cervix of the female rat. This immunoreactivity is probably present in afferent nerves and could play a role in neuroendocrine reflexes and in reproductive function.
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References
Bauer FE, Christofides ND, Hacker GW, Blank MA, Polak JM, Bloom SR (1986) Distribution of galanin immunoreactivity in the genitourinary tract of man and rat. Peptides 7:5–10
Bek T, Ottesen B, Fahrenkrug J (1988) The effect of galanin, CGRP and ANP on spontaneous smooth muscle activity of rat uterus. Peptides 9:497–500
Ch'ng JLC, Christofides ND, Anand P, Gibson SJ, Allen YS, Su HC, Tatemoto K, Morrison JFB, Polak JM, Bloom SR (1985) Distribution of galanin immunoreactivity in the central nervous system and the responses of galanin-containing neuronal pathways to injury. Neuroscience 16:343–354
Dalsgaard C-J, Vincent SR, Schultzberg M, Hökfelt T, Elfvin L-G, Terenius L, Dockray GJ (1983) Capsaicin-induced depletion of substance P-like immunoreactivity of guinea pig sympathetic ganglia. J Auton Nerv Sys 9:595–606
Dunning BE, Ahren B, Veith RC, Bottcher G, Sundler F, Taborsky GJ (1986) Galanin: A novel pancreatic neuropeptide. Am J Physiol 251:E127-E133
Ekblad E, Håkanson R, Sundler F, Wahlestedt C (1985a) Galanin: neuromodulatory and direct contractile effects on smooth muscle preparations. Br J Pharmacol 86:241–246
Ekblad E, Rökaeus A, Håkanson R, Sundler F (1985b) Galanin nerve fibers in the rat gut: distribution, origin and projections. Neuroscience 16:355–363
Furness JB, Costa M, Rökaeus A, McDonald TJ, Brooks B (1987) Galanin-immunoreactive neurons in the guinea-pig small intestine: their projections and relationships to other enteric neurons. Cell Tissue Res 250:607–615
Gamse R (1982) Capsaicin and nociception in the rat and mouse: Possible role of substance P. Naunyn-Schmiedeberg's ArchPharmacol 320:205–216
Ghatei MA, Gu J, Mulderry PK, Blank MA, Allen JM, Morrison JFB, Polak JM, Bloom SR (1985) Calcitonin gene-related peptide (CGRP) in the female rat urogenital tract. Peptides 6:809–815
Gibbins IL, Furness JB, Costa M (1987) Pathway-specific patterns of the co-existence of substance P, calcitonin gene-related peptide, cholecystokinin and dynorphin in neurons of the dorsal root ganglia of the guinea pig. Cell Tissue Res 248:417–437
Grunditz T, Håkanson R, Sundler F, Uddman R (1987) Neurokinin A and galanin in the thyroid gland: Neuronal localization. Endocrinology 12:575–585
Haynes LW (1986) Galanin: Profile of a new neuropeptide. Trends Pharmacol Sci 7:214–215
Hökfelt T, Wiesenfeld-Hallin Z, Villar M, Melander T (1987) Increase of galanin-like immunoreactivity in rat dorsal root ganglion cells after peripheral axotomy. Neurosci Lett 83:217–220
Jancsó N, Jancsó-Gabor A, Szolcsányi J (1967) Direct evidence for neurogenic inflammation and its prevention by denervation and by pretreatment with capsaicin. Br J Pharmacol 31:138–151
Jancsó G, Király E, Jancsó-Gabor A (1977) Pharmacologically induced selective degeneration of chemosensitive primary sensory neurones. Nature 270:741–743
Ju G, Hökfelt T, Brodin E, Fahrenkrug J, Fischer JA, Frey P, Elde RP, Brown JC (1987) Primary sensory neurons of the rat showing calcitonin gene-related peptide immunoreactivity and their relation to substance P-, somatostatin-, galanin-, vasoactive intestinal polypeptideand cholecystokinin-immunoreactive ganglion cells. Cell Tissue Res 247:417–431
Kummer W (1987) Galaninand neuropeptide Y-like immunoreactivities coexist in paravertebral sympathetic neurones of the cat. Neurosci Lett 78:127–131
Lawson SN, Nickels SM (1980) The use of morphometric techniques to analyse the effect of neonatal capsaicin treatment on rat dorsal root ganglia and dorsal roots. J Physiol (Lond) 303:12P
Matsuyama T, Wanaka A, Yoneda S, Kimura K, Kamada T, Girgis S, MacIntyre I, Emson PC, Tohyama M (1986) Two distinct calcitonin gene-related peptide-containing peripheral nervous systems: Distribution and quantitative differences between the iris and cerebral artery with special reference to substance P. Brain Res 373:205–212
Melander T, Hökfelt T, Rökaeus A, Fahrenkrug J, Tatemoto K, Mutt V (1985) Distribution of galanin-like immunoreactivity in the gastro-intestinal tract of several mammalian species. Cell Tissue Res 239:253–270
Melander T, Hökfelt T, Rökaeus A, Cuello AC, Oertel WH, Verhofstad A, Goldstein M (1986) Coexistence of galanin-like immunoreactivity with catecholamines, 5-hydroxytryptamine, GABA, and neuropeptides in the rat CNS. J Neurosci 6:3640–3654
Morris JL, Gibbins IL (1987) Neuronal colocalization of peptides, catecholamines, and catecholamine-synthesizing enzymes in guinea pig paracervical ganglia. J Neurosci 7:3117–3130
Nagy JI, Hunt SP, Iversen LL, Emson PC (1981) Biochemical and anatomical observations on the degeneration of peptidecontaining primary afferent neurons after neonatal capsaicin. Neuroscience 6:1923–1934
Ohhashi T, Jacobowitz DM (1985) Galanin potentiates electrical stimulation and exogenous norepinephrine-induced contractions in the rat vas deferens. Regul Pept 12:163–171
Palmer JM, Schemann M, Tamura K, Wood JD (1986) Galanin mimics slow synaptic inhibition in myenteric neurons. Eur J Pharmacol 124:379–380
Papka RE, Cotton JP, Traurig HH (1985a) Comparative distribution of neuropeptide tyrosine-, vasoactive intestinal polypeptide-, substance P-immunoreactive, acetylcholinesterase-positive and noradrenergic nerves in the reproductive tract of the female rat. Cell Tissue Res 242:475–490
Papka RE, Traurig HH, Wekstein M (1985b) Localization of peptides in nerve terminals in the paracervical ganglion of the rat by light and electron microscopic immunohistochemistry:enkephalin and atrial natriuretic factor. Neurosci Lett 61:285–290
Papka RE, Traurig HH (1987) Substance K-, substance P-, and calcitonin gene-related peptide-immunoreactive nerves in the female reproductive organs. In: Henry JL (ed) Substance P and Neurokinins. Springer, Berlin Heidelberg New York, pp 229–231
Papka RE, Traurig HH (1988) Distribution of subgroups of neuropeptide Y-immunoreactive and noradrenergic nerves in the female rat uterine cervix. Cell Tissue Res 252:533–541
Papka RE, Traurig HH, Klenn P (1987) Paracervical ganglia of the female rat: Histochemistry and immunohistochemistry of neurons, SIF cells, and nerve terminals. Am J Anat 179:243–257
Rattan S, Goyal RK (1987) Effect of galanin on the opossum lower esophageal sphincter. Life Sci 41:2783–2790
Rökaeus A, Melander T, Hökfelt T, Lundberg JM, Tatemoto K, Carlquist M, Mutt V (1984) A galanin-like peptide in the central nervous system and intestine of the rat. Neurosci Lett 47:161–166
Scadding JW (1980) The permanent anatomical effects of neonatal capsaicin on somatosensory nerves. J Anat 131:473–484
Skofitsch G, Jacobowitz DM (1985a) Galanin-like immunoreactivity in capsaicin sensitive sensory neurons and ganglia. Brain Res Bull 15:191–195
Skofitsch G, Jacobowitz DM (1985b) Immunohistochemical mapping of galanin-like neurons in the rat central nervous system. Peptides 6:509–546
Stefanini N, DeMartino C, Zamboni L (1967) Fixation of ejaculated spermatazoa for electron microscopy. Nature (Lond) 216:173–174
Stjernquist M, Ekblad E, Owman Ch, Sundler F (1988) Immunocytochemical localization of galanin in the rat male and female genital tracts and motor effects in vitro. Regul Pept 20:335–343
Strömberg I, Björklund H, Melander T, Rökaeus A, Hökfelt T, Olson L (1987) Galanin-immunoreactive nerves in the rat iris: Alterations induced by denervations. Cell Tissue Res 250:267–275
Su HC, Wharton J, Polak JM, Mulderry PK, Ghatei MA, Gibson SJ, Terenghi G, Morrison JFB, Ballesta J, Bloom SR (1986) Calcitonin gene-related peptide immunoreactivity in afferent neurons supplying the urinary tract: Combined retrograde tracing and immunohistochemistry. Neuroscience 18:727–747
Tamura K, Palmer JM, Wood JD (1987) Galanin suppresses nicotinic synaptic transmission in the myenteric plexus of guinea-pig small intestine. Eur J Pharmacol 136:445–446
Tatemoto K, Rökaeus A, Jornvall H, McDonald TJ, Mutt V (1983) Galanin — a novel biologically active peptide from porcine intestine. FEBS Lett 164:124–128
Traurig HH, Papka RE, Rush M (1987) Role of capsaicin-sensitive primary afferent nerves in the neuroendocrine copulation reflex. In: Henry JL (ed) Substance P and Neurokinins. Springer, Berlin Heidelberg New York, pp 226–228
Traurig HH, Papka RE, Rush ME (1988) Effects of capsaicin on reproductive function in the female rat: role of peptide-containing primary afferent nerves innervating the uterine cervix in the neuroendocrine copulatory response. Cell Tissue Res 253:573–581
Wharton J, Gulbenkian S, Mulderry PK, Ghatei MA, McGregor GP, Bloom SR, Polak JM (1986) Capsaicin induces a depletion of calcitonin gene-related peptide (CGRP)-immunoreactive nerves in the cardiovascular system of the guniea pig and rat. J Auton Nerv Sys 16:289–309
Yau WM, Dorsett JA, Youther ML (1986) Evidence for galanin as an inhibitory neuropeptide on myenteric cholinergic neurons in the guniea pig small intestine. Neurosci Lett 72:305–308
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Papka, R.E., Traurig, H.H. Galanin-immunoreactive nerves in the female rat paracervical ganglion and uterine cervix: distribution and reaction to capsaicin. Cell Tissue Res. 257, 41–51 (1989). https://doi.org/10.1007/BF00221632
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DOI: https://doi.org/10.1007/BF00221632