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The physical location of fourteen RFLP markers in rice (Oryza sativa L.)

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Abstract

A biotin-labeled in situ hybridization technique was used in order to physically map RFLP markers to the chromosomes of rice (Oryza sativa L.). Fourteen RFLP markers, associated with the ends of the linkage groups on rice chromosomes 7, 8, 11, 12, were physically mapped onto specific regions of the chromosomes. The average detection rate of in situ hybridization was 5.91%. The markers were located on seven different chromosome arms. Ten of the fourteen markers were distributed near the chromosome ends. This demonstrated that the RFLP linkage groups involved covered a wide physical distance and that the centromeric region was bisected by all but one linkage group. Two markers covered a short genetic distance but were physically distant, while two covering a longer genetic distance were physically closer together. This indicates that considerable variation can, and does, exist between genetic and physical maps.

This paper is a contribution of the U.S. Department of Agriculture, Agricultural Research Service, and Missouri Agricultural Experiment Station, Journal Series No. 11 882

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Authors and Affiliations

  1. College of Life Science, Wuhan University, 430072, Wuhan Hubei, The People's Republic of China

    Y. C. Song & J. P. Gustafson

  2. Agricultural Research Service, Plant Genetics Research Unit, U.S. Department of Agriculture, 65211, Columbia, MO, USA

    Y. C. Song & J. P. Gustafson

  3. Department of Agronomy, University of Missouri, 65211, Columbia, MO, USA

    Y. C. Song & J. P. Gustafson

Authors
  1. Y. C. Song
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  2. J. P. Gustafson
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Communicated by G. S. Kush

This paper reports the results of research only. Mention of a proprietary product does not constitute an endorsement or a recommendation for its use by the U.S. Department of Agriculture or the University of Missouri

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Song, Y.C., Gustafson, J.P. The physical location of fourteen RFLP markers in rice (Oryza sativa L.). Theoret. Appl. Genetics 90, 113–119 (1995). https://doi.org/10.1007/BF00221003

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  • DOI: https://doi.org/10.1007/BF00221003

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