Summary
By means of the unlabeled antibody peroxidase-antiperoxidase (PAP) technique at the electron microscopic level, it was demonstrated that the hormones of the neural lobe of the rat hypophysis are located in separate neurophysin-vasopressinergic and neurophysin-oxytocinergic nerve fibres. These observations confirm the results of our previous immunocytochemical studies at the light microscopic level.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Acher, R.: Chemistry of the neurohypophysial hormones: an example of molecular evolution. In: Handbook of physiology. Section 7: Endocrinology, Vol. IV. The pituitary gland and its neuroendocrine control, Part 1, pp. 119–130 (R.O. Greep and E.B. Astwood, eds.). Washington: American Physiological Society 1974
Hope, D.B., Pickup, J.C.: Neurophysins. In: Handbook of physiology. Section 7: Endocrinology, Vol. IV. The pituitary gland and its neuroendocrine control, Part 1, pp. 173–189 (R.O. Greep and E.B. Astwood, eds.). Washington: American Physiological Society 1974
Leclerc, R., Pelletier, G.: Electron microscope immunohistochemical localization of vasopressin in the hypothalamus and neurohypophysis of the normal and Brattleboro rat. Amer. J. Anat. 140, 583–588 (1974)
Lederis, K.: Neurosecretion and the functional structure of the neurohypophysis. In: Handbook of physiology. Section 7: Endocrinology, Vol. IV. The pituitary gland and its neuroendocrine control, Part 1, pp. 81–102 (R.O. Greep and E.B. Astwood, eds.). Washington: American Physiological Society 1974
Mey, J. de, Dierickx, K., Vandesande, F.: Identification of neurophysin producing cells. III. Immunohistochemical demonstration of neurophysin I-producing neurons in the bovine infundibular nucleus. Cell Tiss. Res. 161, 219–224 (1975)
Mey, J. de, Vandesande, F., Dierickx, K.: Identification of neurophysin producing cells. II. Identification of the neurophysin I and the neurophysin II producing neurons in the bovine hypothalamus. Cell Tiss. Res. 153, 531–543 (1974)
Moriarty, G.C.: Adenohypophysis: Ultrastructural cytochemistry. A review. J. Histochem. Cytochem. 21, 855–894 (1973)
Moriarty, G.C., Halmi, N.S.: Electron microscopic study of the adrenocorticotropin-producing cell with the use of unlabeled antibody and the soluble peroxidase-antiperoxidase complex. J. Histochem. Cytochem. 20, 590–603 (1972)
Moriarty, G.C., Moriarty, C.M., Sternberger, L.A.: Ultrastructural immunocytochemistry with unlabeled antibodies and the peroxidase-antiperoxidase complex. A technique more sensitive than radio-immunoassay. J. Histochem. Cytochem. 21, 825–833 (1973)
Pelletier, G., Leclerc, R., Labrie, F., Puviani, R.: Electron microscope immunohistochemical localization of neurophysin in the rat hypothalamus and pituitary. Molec. Cell Endocr. 1, 157–166 (1974)
Silverman, A.J., Knigge, K.M., Zimmerman, E.A.: Ultrastructural immunocytochemical localization of neurophysin in freeze-substituted neurohypophysis. Amer. J. Anat. 142, 265–271 (1975)
Silverman, A.J., Zimmerman, E.A.: Ultrastructural immunocytochemical localization of neurophysin and vasopressin in the median eminence and posterior pituitary of the guinea pig. Cell Tiss. Res. 159, 291–301 (1975)
Sternberger, L.A., Hardy, P.H. Jr., Cuculis, J.J., Meyer, H.G.: The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J. Histochem. Cytochem. 18, 315–333 (1970)
Swaab, D.F., Pool, C.W.: Specificity of oxytocin and vasopressin immunofluorescence. J. Endocr. 66, 263–272 (1975)
Swaab, D.F., Pool, C.W., Nijveldt, F.: Immunofluorescence of vasopressin and oxytocin in the rat hypothalamo-neurohypophyseal system. J. Neur. Trans. 36, 195–215 (1975)
Vandesande, F., Dierickx, K.: Identification of the vasopressin producing and of the oxytocin producing neurons in the hypothalamic magnocellular neurosecretory system of the rat. Cell Tiss. Res. 164, 153–162 (1975)
Vandesande, F., Dierickx, K., Mey, J. de: Identification of the vasopressin-neurophysin II and the oxytocin-neurophysin I producing neurons in the bovine hypothalamus. Cell Tiss. Res. 156, 189–200 (1975)
Vandesande, F., Dierickx, K.: Immuno-cytochemical demonstration of the inability of the homozygous Brattleboro rat to synthesize vasopressin and vasopressin-associated neurophysin. Cell Tiss. Res. 165, 307–316 (1976)
Author information
Authors and Affiliations
Additional information
This investigation was supported by a grant from the Belgian Nationaal Fonds voor Geneeskundig Wetenschappelijk Onderzoek
Rights and permissions
About this article
Cite this article
Aspeslagh, M.R., Vandesande, F. & Dierickx, K. Electron microscopic immunocytochemical demonstration of separate neurophysin-vasopressinergic and neurophysin-oxytocinergic nerve fibres in the neural lobe of the rat hypophysis. Cell Tissue Res. 171, 31–37 (1976). https://doi.org/10.1007/BF00219698
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00219698