Summary
The ultrastructure of endocrine-like cells of the human lung was compared to the ultrastructure of endocrine-like cells of the stomach and pancreas in both adult and foetal material.
Three types of endocrine-like cells were found in the human foetal lung. Type 1 or P1 cells contained very small granules (about 110 nm) of two varieties, cored and vesicular; type 2 or P2 cells with cored granules measuring about 130 nm; and type 3 cells with cored granules of about 180–190 nm. In the adult lung only one type Pa cells with cored granules could be found.
Cells resembling foetal P1 cells were not found in foetal or adult gastric mucosa, or in the pancreas. In the gastric mucosa cells resembling pulmonary Pa or P2 cells were moderately represented and often difficult to distinguish from each other. Thus, they were grouped together as gastric P cells. Cells with granules resembling those of pulmonary type 3 cells were found most numerous in the adult oxyntic mucosa. Cells resembling gastric P cells (and pulmonary P2 cells) were rather numerous in foetal pancreas, but very rare in adult pancreas. Few cells containing granules somewhat resembling those of pulmonary type 3 cells were present in both foetal and adult pancreas.
The results were discussed in respect to 1) the similarities between some gastric or pancreatic carcinoids and lung carcinoids, 2) the gastro-pancreatic P cells as a separate cell population, 3) the possible secretion by the lung endocrine-like cells of active substances, either amines or peptides, 4) the similarity between the secretory granules of Pa and P1 cells and neurosecretory granules of the hypothalamus and between P2 cells and some endocrine cells of the pituitary.
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References
Allison, D.J., Clay, T.: Angiotensin II release from the hypoxic lobe of the intact dog lung. J. Physiol. (Lond.) 260, 32P-33P (1976)
Arias-Stella, J., Valcarcel, J.: Chief cell hyperplasia in the human carotid body at high altitudes. Physiologic and pathologic significance. Human Path. 7, 361–373 (1976)
Basset, F., Poirier, J, Le Crom, M., Turiaf, J.: Etude ultrastructurale de lépithélium bronchiolaire humain. Z. Zellforsch. 116, 425–442 (1971)
Beck, C., Larkins, R.G., Martin, T.J., Burger, H.G.: Stimulation of growth hormone release from superfused rat pituitary by extracts of hypothalamus and of human lung tumours. J. Endocr. 59, 325–333 (1973)
Bensch, K.G., Gordon, G.B., Miller, L.R.: Studies on the bronchial counterpart of the Kultschitzky (argentaffin) cell and innervation of bronchial glands. J. Ultrastruct. Res. 12, 668–686 (1965)
Bensch, K.G., Gordon, G.B., Miller, L.R.: Electron microscopic and biochemical studies on the bronchial carcinoid tumour. Cancer (Philad.) 18, 592–602 (1965)
Bergland, R.M., Torack, R.: An electron microscopic study of the human infundibulum. Z. Zellforsch. 99, 1–12 (1969)
Berkov, S.: Hypoxic pulmonary vasoconstriction in the rat. The necessary role of angiotensin II. Circulat. Res. 35, 256–261 (1974)
Black, W.C.: Enterochromaffin cell types and corresponding carcinoid tumours. Lab. Invest. 19, 473–486 (1968)
Bonikos, D.S., Archibald, R., Bensch, K.G.: On the origin of the so-called tumorlets of the lung. Human Path. 7, 461–469 (1976)
Buffa, R., Capella, C., Solcia, E., Frigerio, B., Said, S.I.: Vasoactive intestinal peptide (VIP) cells in the pancreas and gastro-intestinal mucosa. An immunohistochemical and ultrastructural study. Histochemistry 50, 217–227 (1977)
Capella, C., Solcia, E.: Optical and electron microscopical study of cytoplasmic granules in human carotid body, carotid body tumours and glomus jugulare tumours. Virchows Arch. Abt. B 7, 37–53 (1971)
Capella, C., Solcia, E., Frigerio, B., Buffa, R.: Endocrine cells of the human intestine. An ultrastructural study. In: Endocrine gut and pancreas (T. Fujita, ed.), pp. 42–59. Amsterdam: Elsevier 1976
Capella, C., Solcia, E., Frigerio, B., Buffa, R., Usellini, L., Fontana, P.: The endocrine cells of the pancreas and related tumours. Ultrastructural study and classification. Virchows Arch. Abt. A 373, 327–352 (1977)
Capella, C., Vassallo, G., Solcia, E.: Light and electron microscopic identification of the histamine-storing argyrophil (ECL) cell in murine stomach and of its equivalent in other mammals. Z. Zellforsch. 118, 68–84 (1971)
Churg, A., Warnock, M.L.: Pulmonary tumorlets. A form of peripheral carcinoid. Cancer (Philad.) 37, 1469–1477 (1976)
Dabek, J.T.: Bronchial carcinoid tumour with acromegaly in two patients. J. clin. Endocr. 28, 329–333 (1974)
Ericson, L.E., Håkanson, R., Larsson, B., Owman, Ch., Sundler, F.: Fluorescence and electron microscopy of amine-storing enterochromaffin-like cells in tracheal epithelium of mouse. Z. Zellforsch. 124, 532–545 (1972)
Erspamer, V., Melchiorri, P.: Actions of bombesin on secretions and motility of the gastrointestinal tract. In: Gastrointestinal Hormones (J.C. Thompson, ed.), pp. 575–589. Austin and London: University of Texas Press 1975
Forssmann, A.: The ultrastructure of the cell types in the endocrine pancreas of the horse. Cell Tiss. Res. 167, 179–195 (1976)
Gabrielli, M., Capella, C., Bordi, C., Buffa, R., Solcia, E.: Bronchial carcinoid. Ultrastructural characterization of different types. (1978, in preparation)
George, J.M., Capen, C.C., Phillips, A.S.: Biosynthesis of vasopressin in vitro and ultrastructure of a bronchogenic carcinoma. J. clin. Invest. 51, 141–148 (1972)
Gewirtz, G., Yalow, R.S.: Ectopic-ACTH production in carcinoma of the lung. J. clin. Invest. 53, 1022–1032 (1974)
Häggendal, J.: The presence of dopamine in human gastric juice. Acta physiol. scand. 71, 127–128 (1967)
Hage, E.: Endocrine cells in bronchial mucosa of human foetuses. Acta path. microbiol. scand. (A) 80, 225–234 (1972)
Hage, E.: Electron microscopic identification of several types of endocrine cells in the bronchial epithelium of human foetuses. Z. Zellforsch. 141, 401–412 (1973)
Hage, E.: Histochemistry and fine structure of bronchial carcinoid tumours. Virchows Arch. Abt. A 361, 121–128 (1973)
Hage, E.: Endocrine-like cells of the pulmonary epithelium. In: Chromaffin, enterochromaffin and related cells (R.E. Coupland and T. Fujita, eds.), pp. 317–332 Amsterdam: Elsevier 1976
Hage, E., Hage, J., Juel, G.: Endocrine-like cells of the pulmonary epithelium of the human adult lung. Cell Tiss. Res. 178, 39–48 (1977)
Håkanson, R., Sjöberg, N.-O., Sporrong, B.: Amine mechanisms in enterochromaffin and enterochromaffin-like cells of gastric mucosa of various mammals. Histochemie 21, 189–220 (1970)
Holdaway, I.M., Bloomfield, G.A., Ratcliffe, J.G., Hinson, K.W.F., Rees, G.M., Rees, L.H.: Adenocorticotrophin levels in normal and neoplastic lung tissue. In: Endocrinology 1973 (S. Taylor, ed.), pp. 309–315. London: Heinemann 1974
Hosoda, S., Nakamura, W., Suzuki, H., Hoshino, M., Karasawa, K.: A bronchial carcinoid having low serotonin concentration. Arch. Path. 90, 320–324 (1970)
Imura, H., Matsukura, S., Yamamoto, H., Hirata, Y., Nakai, Y., Endo, J., Tanaka, A., Nakamura, M.: Studies on ectopic ACTH-producing tumors. II Clinical and biochemical features of 30 cases. Cancer (Philad.) 35, 1430–1437 (1975)
Larsson, L.-I., Sundler, F., Håkanson, R.: Pancreatic polypeptide — A postulated new hormone: Identification of its cellular storage site by light and electron microscopy immunocytochemistry. Diabetologia 12, 211–226 (1976)
Lauweryns, J.M., Cokelaere, M.: Intrapulmonary neuroepithelial bodies: hypoxiasensitive neuro-(chemo-) receptors. Experientia (Basel) 29, 1384–1386 (1973)
Lauweryns, J.M., Cokelaere, M., Theunynck, P.: Neuroepithelial bodies in the respiratory mucosa of various mammals. A light optical, histochemical and ultrastructural investigation. Z. Zellforsch. 135, 569–592 (1972)
Lauweryns, J.M., Peuskens, J., Cokelaere, M.: Argyrophil, fluorescent and granulated (peptide and amine producing?) AFG cells in human infant bronchial epithelium; light and electron microscopic studies. Life Sci. 9, 1417–1429 (1970)
Libet, B., Owman, Ch.: Concomitant changes in formaldehyde-induced fluorescence of dopamine interneurons and in slow inhibitory postsynaptic potentials of the rabbit superior cervical ganglion, induced by stimulation of the preganglionic nerve or by a muscarinic agent. J. Physiol. (Lond.) 237, 635–662 (1974)
Moller, M., Mollgard, K., Sorensen, S.C.: The ultrastructure of the carotid body in chronically hypoxic rabbits. J. Physiol. (Lond.) 238, 447–453 (1974)
Orci, L.: Cells containing somatostatin in the pancreas and the gastrointestinal tract. In: Polypeptide hormones: molecular and cellular aspects, pp. 313–323, Ciba Foundation Symp. 41 Amsterdam: Elsevier 1976
Pariente, R., Even, P., Brouet, G.: Etude ultrastructurale des carcinoides bronchiques. Presse méd. 75, 183–188 and 221–224 (1967)
Patchefsky, A.S., Gordon, G., Harrer, W.V., Hoch, W.S.: Carcinoid tumor of the pancreas. Ultrastructural observations of a lymph node metastasis and comparison with bronchial carcinoid. Cancer (Philad.) 33, 1349–1354 (1974)
Rubin, W.: A fine structural characterization of the proliferated endocrine cells in atrophic gastric mucosa. Amer. J. Paht. 70, 109–118 (1973)
Said, S.I.: Endocrine role of the lung in disease. Amer. J. Med. 57, 453–465 (1974)
Said, S.I., Faloona, G.R.: Elevated plasma and tissue levels of vasoactive intestinal polypeptide in the watery diarrhea syndrome due to pancreatic, bronchogenic and other tumours. New Engl. J. Med. 293, 155–160 (1975)
Said, S.I., Mutt, V.: Long acting vasodilator peptide from lung tissues. Nature (Lond.) 224, 699–700 (1969)
Sönksen, P.M., Ayres, A.B., Braimbridge, M., Corrin, B., Davies, D.R., Jeremiah, G.M., Oaten, S.W., Lowy, C., West, T.E.T.: Acromegaly caused by pulmonary carcinoid tumours. Clin. Endocr. 5, 503–513 (1976)
Solcia, E., Capella, C., Buffa, R., Frigerio, B., Fontana, P., Usellini, L.: Tumori dell'adenoipofisi: Diagnosi morfologica e classificazione. Pathologica (Genova) 69, 333–346 (1977)
Solcia, E., Capella, C., Vassallo, G., Buffa, R.: Endocrine cells of the gastric mucosa. Int. Rev. Cytol. 42, 223–286 (1975)
Terzakis, J.A., Sommers, S.C., Andersson, B.: Neurosecretory appearing cells of human segmental bronchi. Lab. Invest. 26, 127–132 (1972)
Upton, G.V., Amatruda, T.T.: Evidence for the presence of tumor peptides with corticotrophinreleasing factor-like activity in the ectopic ACTH syndrome. New Engl. J. Med. 285, 419–424 (1971)
Vassallo, G., Capella, C., Solcia, E.: Endocrine cells of the human gastric mucosa. Z. Zellforsch. 118, 49–67 (1971)
Vassallo, G., Solcia, E., Bussolati, G., Polak, J., Pearse, A.G.E.: Non G cell gastrin producing tumors of the pancreas. Virchows Arch. Abt. B 11, 66–79 (1972)
Wilander, E., Westermark, P.: On argyrophil reactions of endocrine cells in the human fetal pancreas. Cell Tiss. Res. 168, 33–43 (1976)
Williams, E.D., Sandler, M.: The classification of carcinoid tumours. Lancet 1963 I, 238–239
Yasuda, N., Greer, M.A.: Demonstration of corticotrophin-releasing activity in rat and human peripheral blood. Acta endocr. (Kbh.) 84, 1–10 (1977)
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Supported in part by the Italian National Research Council (Grants N. 75.00630.04 and N. 76.01558.04)
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Capella, C., Hage, E., Solcia, E. et al. Ultrastructural similarity of endocrine-like cells of the human lung and some related cells of the gut. Cell Tissue Res. 186, 25–37 (1978). https://doi.org/10.1007/BF00219652
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DOI: https://doi.org/10.1007/BF00219652