Summary
Qualitative and quantitative studies were made to determine the amount of nerve fiber supplying corpora lutea (CL) of rats during the oestrous cycle and pregnancy and sow CL during days 4–6 after ovulation. Fluorescence microscopy of freeze-dried, paraformaldehyde treated (Falck-Hillarp method) rat ovaries reveals adrenergic nerve fibers which run along with vessels and form a network among interstitial gland cells. Nerve fibers do not enter the granulosa cell layer in follicles or CL. In the CL circumference both vascular and non-vascular nerves occur the latter being related to the fibromuscular layer and probably innervating smooth muscle cells. No striking differences exist between the innervation of the ovary in non-pregnant and pregnant rats. Bodian and methylene blue staining did not contribute to a more detailed knowledge of rat ovary nerve supply. Electron microscopic quantitative analysis of rat and pig CL (rat: day 18 of pregnancy; pig: day 4–6 after ovulation) revealed no axon profiles in 2.000 grid squares (one square measuring 2.25×10-2 mm2) of randomly taken CL sections. Thus it was possible to calculate an upper limit of 133 μm of nerve fibers per 1 mm3 CL tissue, in case there were any at all.
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Axelsson, S., Björklund, A., Falck, B., Lindvall, O., Svensson, L.-Å.: Glyoxylic acid condensation: a new fluorescence method for the histochemical demonstration of biogenic monoamines. Acta physiol. scand. 87, 57–62 (1973)
Bahr, J., Kao, L., Nalbandov, A, V.: The role of catecholamines and nerves in ovulation. Biol. Reprod. 10, 273–290 (1974)
Bargmann, W.: On the innervation of vertebrate endocrine organs. In: Endocrinology (ed. O. Scow), Proc. IV. Congress Endocrinol. Washington 1972, p. 220–223. Amsterdam: Excerpta Medica; New York: American Elsevier Publ. Co. 1973
Bjersing, L.: On the ultrastructure of granulosa lutein cells in porcine corpus luteum. With special reference to endoplasmic reticulum and steroid hormone synthesis. Z. Zellforsch. 82, 187–211 (1967)
Björklund, A., Falck, B., Owman, Ch.: Fluorescence microscopic and microspectrofluorometric techniques in the cellular localization and characterization of biogenic amines. In: S. A. Berson (ed.), Methods of investigative and diagnostic endocrinology, vol. 1: J. E. Rall and I. J. Kopin (eds.), The thyroid and biogenic amines, p. 318–368. Amsterdam: North Holland Publ. Co. 1972
Björklund, A., Lindvall, O., Svensson, L.-Å.: Mechanisms of fluorophore formation in the histochemical glyoxylic acid method for monoamines. Histochemie 32, 113–131 (1972)
Burden, H. W.: Adrenergic innervation in ovaries of the rat and guinea pig. Amer. J. Anat. 133, 455–462 (1972)
Corner, G. W.: The corpus luteum of pregnancy, as it is in swine. Contr. Embryol. Carneg. Inst. 222, 69–94
Corner, G. W.: On the origin of the corpus luteum of the sow from both granulosa and theca interna Amer. J. Anat. 26, 117–183 (1919)
Corner, G. W.: Cyclic changes in the ovaries and uterus of the sow, and their relation to the mechanism of implantation. Contr. Embryol. Carneg. Inst. 13, 117–146 (1921)
Dahl, E.: Studies on the fine structure of ovarian interstitial tissue. 3. The innervation of the thecal gland of the domestic fowl. Z. Zellforsch. 109, 212–226 (1970)
Falck, B., Hillarp, N.-Å., Thieme, G., Torp, A.: Fluorescence of catechol amines and related compounds condensed with formaldehyde. J. Histochem. Cytochem. 10, 348–354 (1962)
Fuxe, K., Hökfelt, T., Jonsson, G., Ungerstedt, U.: Fluorescence microscopy in neuroanatomy. In: S. J. Nauta and S.O.E. Ebbesson (eds.), Contemporary research methods in neuroanatomy, p. 275–314. Berlin-Heidelberg-New York: Springer 1970
Hennig, A.: Länge eines dreidimensionalen Linienzuges. Proc. I. Internat. Congr. Stereology 44, 1–8 (1963)
Kayanja, F.I.B., Naeves, W.B.: The fine structure of the corpus luteum in Hyrax. Z. Zellforsch. 144, 475–487 (1973)
Lindvall, O., Björklund, A., Hökfelt, T., Ljungdahl, Å.: Application of the glyoxylic acid method to vibratome sections for the improved visualization of central catecholamine neurons. Histochemie 35, 31–38 (1973)
Long, J. A.: Corpus luteum of pregnancy in the rat—ultrastructural and cytochemical observations. Biol. Reprod. 8, 87–99 (1973)
Mossman, H. W., Duke, K. L.: Comparative morphology of the mammalian ovary. The University of Wisconsin Press, 1973
O'Donoghue, P. N.: Reproduction in the female hyrax (Dendrohyrax arborea ruwenzorii). Proc. zool. Soc. London 141, 207–237 (1963)
Owman, Ch., Svensson, K.-G.: Personal communication (1974a)
Owman, Ch., Svensson, K.-G.: Personal communication (1974b)
Rosengren, E., Sjöberg, N. O.: Changes in the amount of adrenergic transmitter in the female genital tract of the rabbit during pregnancy. Acta physiol. scand. 72, 412–424 (1968)
Stöhr jr., Ph.: Mikroskopische Anatomie des vegetativen Nervensystems. In: Handbuch der mikroskopischen Anatomie des Menschen, Bd. IV, Teil 5. Berlin-Göttingen-Heidelberg: Springer 1957
Unsicker, K.: Zur Innervation der Nebennierenrinde vom Goldhamster. Eine fluoreszenzund elektronenmikroskopische Studie. Z. Zellforsch. 95, 608–619 (1969)
Unsicker, K.: Zur Innervation der interstitiellen Drüse im Ovar der Maus (Mus musculus L.). Z. Zellforsch. 109, 46–54 (1970)
Unsicker, K.: On the innervation of the rat and pig adrenal cortex. Z. Zellforsch. 115, 151–156 (1971)
Unsicker, K.: Innervation of the testicular interstitial tissue in reptiles. Z. Zellforsch. 146, 123–138 (1973a)
Unsicker, K.: Fine structure and innervation of the avian adrenal gland. V. Innervation of interrenal cells. Z. Zellforsch. 146, 403–416 (1973b)
Unsicker, K.: Innervation of adrenal cells in the lizards Lacerta dugesi und Lacerta pityusensis. Gen. comp. Endocr. (in press)
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Supported by a grant from the „Deutsche Forschungsgemeinschaft” (Un 34/1).
I am much obliged to Mrs. K. Jacob, Mrs. A. Löwe, and Mrs. R. Sprang for valuable technical assistance.
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Unsicker, K. Qualitative and quantitative studies on the innervation of the corpus luteum of rat and pig. Cell Tissue Res. 152, 513–523 (1974). https://doi.org/10.1007/BF00218935
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DOI: https://doi.org/10.1007/BF00218935