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Peptidergic regulation of secretory activity in amphibian olfactory mucosa: Immunohistochemistry, neural stimulation, and pharmacology

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Summary

The role of substance P in the regulation of secretion from sustentacular cells, Bowman's glands and deep glands in the amphibian olfactory mucosa was investigated using immunohistochemical, electrophysiological, and pharmacological methods. Substance P-like immunoreactive varicose fibers extended through the olfactory epithelium, terminating at or near the surface. In addition, immunoreactive varicose fibers innervated Bowman's glands, deep glands, and blood vessels in the lamina propria. Innervation of Bowman's gland was sparse, with fibers terminating on basal acinar cell membranes; deep gland innervation was abundant, with fibers often extending between acinar cells almost to the lumen. Stimulation of the ophthalmic branch of the trigeminal nerve resulted in slow potentials recorded at the surface of the olfactory epithelium. When the olfactory mucosae from trigeminal-stimulated animals were examined histologically, morphological signs of secretory activity were observed, suggesting that substance P was released from the trigeminal nerve terminals. Topical application of 10-5 to 10-3 mol substance P resulted in morphological signs of secretion that were very similar to those seen as a result of trigeminal stimulation. Thus, substance P released from trigeminal fibers may modulate secretory activity within the olfactory mucosa.

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Authors and Affiliations

  1. Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan, USA

    M. L. Getchell Ph.D. & T. V. Getchell

  2. Laboratoire de Physiologie Neurosensorielle associé au CNRS, Université Claude-Bernard, Villeurbanne, France

    J.-F. Bouvet & A. Holley

  3. Rocky Mountain Taste and Smell Center and Department of Cellular and Structural Biology; University of Colorado Health Sciences Center, Denver, Colorado, USA

    T. E. Finger

Authors
  1. M. L. Getchell Ph.D.
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  2. J.-F. Bouvet
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  3. T. E. Finger
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  4. A. Holley
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  5. T. V. Getchell
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Getchell, M.L., Bouvet, JF., Finger, T.E. et al. Peptidergic regulation of secretory activity in amphibian olfactory mucosa: Immunohistochemistry, neural stimulation, and pharmacology. Cell Tissue Res. 256, 381–389 (1989). https://doi.org/10.1007/BF00218896

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