Summary
Pig spleens were selectively perfused with a fluorescein isothiocyanate (FITC) containing medium. Histological sections of spleens excised one day later revealed the structure of the reticulum in thick cryostat sections directly in three dimensions. The pattern of the reticulum in silver impregnated sections was comparable to the FITC labelling pattern. This technique preferentially outlines the reticulum of the white pulp and the marginal zone of the spleen.
Similar content being viewed by others
References
Blue J, Weiss L (1981) Species variation in the structure and function of the marginal zone — an electron microscope study of cat spleen. Am J Anat 161:169–187
Ford WL, Smith ME (1979) Lymphocyte recirculation between the spleen and the blood. In: Role of the spleen in the immunology of parasitic dieseases. Tropical diseases research series 1. Schwabe, Basel, p 29
Herman PG (1980) Microcirculation of organized lymphoid tissues. Monogr Allergy 16:126–142
Humphrey JH (1981) Differentiation of function among antigen-presenting cells. Ciba Found Symp 84:302–321
Kumararatne DS, MacLennan ICM (1981) Cells of the marginal zone of the spleen are lymphocytes derived from recirculating precursors. Eur J Immunol 11:865–869
Kumararatne DS, Bazin H, MacLennan ICM (1981) Marginal zones: the major B cell compartment of rat spleens. Eur J Immunol 11:858–864
Movat HZ (1961) Silver impregnation methods for electron microscopy. Am J Clin Pathol 35:528–537
Pabst R, Binns RM (1981) In vivo labelling of the spleen and mesenteric lymph nodes with fluorescein isothiocyanate for lymphocyte migration studies. Immunology 44:321–329
Pabst R, Geisler R (1981) The route of migration of lymphocytes from blood to spleen and mesenteric lymph nodes in the pig. Cell Tissue Res 221:361–370
Pabst R, Reilmann H, Neuhaus R (1980) Selective labelling of lymphoid tissues by extracorporeal perfusion. J Immunol Meth 33:23–32
Sasou S, Satodate R, Suzuki A (1980) A scanning electron microscopic study of the perifollicular region of the rat spleen. J Reticuloendothel Soc 27:461–469
Snook T (1964) Studies on the perifollicular region of the rat's spleen. Anat Rec 148:149–160
Weiss LP (1972) The cells and tissues of the immune system. Structure, functions, interactions. Englewood Cliffs. Prentice Hall
Author information
Authors and Affiliations
Additional information
We are grateful to Ms. M. Kaatz and H. Wilting and to Mr. I. King and J. Jarvis for preparing the histological sections and Mr. R. Gallup for the photographs and to Sheila Fryk for secretarial assistance
This study was partly supported by the Deutsche Forschungsgemeinschaft (Pa 240/2)
Rights and permissions
About this article
Cite this article
Pabst, R., Binns, R.M. The three-dimensional reticular structure of the pig spleen demonstrated by labelling with fluorescein isothiocyanate. Cell Tissue Res. 226, 319–325 (1982). https://doi.org/10.1007/BF00218362
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00218362