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Purification and characterization of metallothionein from liver of cadmium exposed Rhesus monkeys (Macaca mulatta)

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Abstract

Metallothionein (MT) a low molecular weight, Cd-binding, cysteine rich, cytosolic protein has been isolated, purified and characterized from cadmium exposed Rhesus monkeys maintained on protein calorie malnourished (PCM) diet. Metallothionein was resolved into three isoforms i.e. MTa, MTb and MTVc. The ratio of Cd, Zn and Cu varied in these isometallothioneins. MTc was the major isometallothionein. UV Spectra of MTc revealed the presence of mercaptide bonds and absence of aromatic amino acids. These observations were further confirmed by amino acid analysis of MTc which demonstrated high cysteine content (22.6) followed by serine, glycine and lysine. The molecular weight of MTc as determined by gel filtration and amino acid analysis was 13000 and 6398 daltons respectively. This demonstrates that MTc is a non-globular ellipsoid polypeptide. MTc showed a unique property of binding selenium. Monkey liver metallothionein was immunologically identical with human metallothionein. All the characteristics of MTc obtained in the present study reveal a similarity between monkey and human metallothionein probably due to closer phylogenetic relationship between the two species.

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Authors and Affiliations

  1. Department of Biochemistry, Postgraduate Institute of Medical Education and Research, 160012, Chandigarh, India

    Vipin K. Paliwal, Krishan K. Kohli, Mridula Sharma & Ravindra Nath

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  1. Vipin K. Paliwal
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  2. Krishan K. Kohli
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  3. Mridula Sharma
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  4. Ravindra Nath
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Paliwal, V.K., Kohli, K.K., Sharma, M. et al. Purification and characterization of metallothionein from liver of cadmium exposed Rhesus monkeys (Macaca mulatta). Mol Cell Biochem 71, 139–147 (1986). https://doi.org/10.1007/BF00214773

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  • DOI: https://doi.org/10.1007/BF00214773

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