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Somaclonal variant UC-T3: the expression of Fusarium wilt resistance in progeny arrays of celery, Apium graveolens L.

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Summary

First generation (S1) progeny, second generation (S2) progeny, and backcross (BC) progeny of a celery (Apium graveolens L. var. dulce) somaclonal variant, UC-T3, were evaluated for resistance to the fungus Fusarium oxysporum f. sp. apii, race 2 (FOA2). Chisquare analysis of S1 progeny showed that the expression of resistance did not fit a single-locus model. S2 progeny means were similar among families, except in a heavily infested field. The lowest ranking S2 family in both the lightly infested and heavily infested fields was significantly more resistant to FOA2 than individuals of the susceptible progenitor line ‘Tall-Utah 527OR’; therefore; it was concluded that the trait was heritable. The phenotypic distribution of the backcross progeny was broad, suggesting that the new resistance was conferred by at least two genes whose expression was dominant to susceptibility. The mean scores for disease resistance of the progeny of crosses between UC-T3 and the moderately resistant line, ‘Tall-Utah 527OHK’, generally equaled the resistance found among the progeny of the most resistant parent.

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References

  • Al-Abta S, Collin HA (1978) Control of embryoid development in tissue cultures of celery. Ann Bot 42:773–782

    Google Scholar 

  • Charudattan R, DeVay JE (1972) Common antigens among varieties of Gossypium hirsutum and isolates of Fusarium and Verticillium species. Phytopathology 62:230–234

    Google Scholar 

  • Chen CH (1976) Vegetative propagation of the celery plant by tissue culture. Proc SD Acad Sci 55:44–48

    Google Scholar 

  • Daub M (1986) Tissue culture and the selection of resistance to pathogens. Annu Rev Phytopathol 24:159–186

    Google Scholar 

  • Dunstan DI, Short KC, Merrick ME, Collin HA (1982) Origin and early growth of celery embryoids. New Phytol 91:121–128

    Google Scholar 

  • Earle ED, Gracen VE, Yoder OC, Germmill KP (1978) Cytoplasm-specific effects of Helminthosporium maydis race T toxin on survival of corn mesophyll protoplasts. Plant Physiol 61:420–424

    Google Scholar 

  • Evans DA, Sharp WR (1983) Single gene mutations in tomato plants regenerated from tissue culture. Science 221:949–951

    Google Scholar 

  • Evans DA, Sharp WR, Medina, Filho HP (1984) Somaclonal and gametoclonal variation. Am J Bot 71:759–774

    Google Scholar 

  • Heath-Pagliuso S, Pullman J, Rappaport L (1988) Somaclonal variation in celery: screening for resistance to Fusarium oxysporum f. sp. apii. Theor Appl Genet 75:446–451

    Google Scholar 

  • Heath-Pagliuso S, Pullman J, Rappaport L (1989) ‘UC-T3 somaclone’: celery germplasm resistant to Fusarium oxysporum f. sp. apii, race 2. Hort Science 24:711–712

    Google Scholar 

  • Larkin PJ, Scowcroft WR (1981) Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197–214

    Google Scholar 

  • Larkin PJ, Ryan SA, Brettell RIS, Scowcroft WR (1984) Heritable Somaclonal variation in wheat. Theor Appl Genet 67:443–455

    Google Scholar 

  • Lee M, Phillips RL (1988) The chromosomal basis of somaclonal variation. Annu Rev Plant Physiol, Plant Mol Biol 39:413–437

    Google Scholar 

  • McNay JW, Chourey PS, Pring DR (1984) Molecular analysis of genomic stability of mitochondrial DNA in tissue-cultured cells of maize. Theor Appl Genet 67:433–437

    Google Scholar 

  • Nadel BL, Altman A, Ziv M (1989) Regulation of somatic embryogenesis in celery cell suspensions. I. Promoting effects of mannitol on somatic embryo development. Plant Cell Tiss Org Cult 18:181–189

    Google Scholar 

  • Orton TJ (1984) Genetic variation in somatic tissues: method or madness? Adv Plant Pathol 2:153–189

    Google Scholar 

  • Orton TJ, Durgan ME, Hulbert SH (1984) Studies on the inheritance of resistance to Fusarium oxysporum f. sp. apii in celery. Plant Dis 68:574–578

    Google Scholar 

  • Pullman GS, Rappaport L (1983) Tissue culture induced variation in celery for Fusarium yellows. Phytopathology (Abstr) 73:818

    Google Scholar 

  • Quiros CF (1987) Breeding celery for disease resistance and improved quality. In: Annual report, California Celery Growers Assn, Dinuba/CA, pp 19–22

    Google Scholar 

  • Rappaport L (1982) Cloning celery for plant propagation. In: Annual report, California Celery Growers Assn, Dinuba/ CA, pp 25–36

    Google Scholar 

  • Rappaport L, Heath-Pagliuso S (1986) Cloning celery for plant propagation. In: Annual report, California Celery Growers Assn, Dinuba/CA, pp 6–11

    Google Scholar 

  • Rappaport L, Heath-Pagliuso S (1987) Cloning celery for plant propagation. In: Annual report, California Celery Growers Assn, Dinuba/CA, pp 25–31

    Google Scholar 

  • Rappaport L, Heath-Pagliuso S, Pullman G, Matlin S, Fuji D (1988) Somaclonal variation as a genetic source of resistance to Fusarium wilt of celery: a case history. In: Application of Biotechnology in Agriculture. Li Xu Hsien eds. Shanghai Scientific and Technical Publishers, Shanghai, P.R.C. pp 137–154

    Google Scholar 

  • Schneider RW (1984) Effects of nonpathogenic strains of Fusarium oxysporum on celery root infection by F. oxysporum f. sp. apii and a novel use of the Lineweaver-Burk double reciprocal plot technique. Phytopathology 74:646–653

    Google Scholar 

  • Scowcroft WR, Larkin PI Brettell RIS (1983) Genetic variation from tissue culture. In: Helgeson JP, Deverall BJ (eds) Use of tissue culture and protoplasts in plant pathology. Academic Press, New York, pp 139–162

    Google Scholar 

  • Shepard JF (1981) Protoplasts as sources of disease resistance in plants. Annu Rev Phytopathol 19:145–166

    Google Scholar 

  • Singer SR, McDaniel CN (1984) Selection of amitrole-tolerant tobacco calli and the expression of this tolerance in regenerated plants and progeny. Theor Appl Genet 67:427–432

    Google Scholar 

  • Steel RGD, Torrie JH (1960) Principles and procedures of statistics. McGraw-Hill, New York pp 72–75 and 113–114

    Google Scholar 

  • Williams L, Collin HA (1976) Growth and cytology of celery plants derived from tissue cultures. Ann Bot 40:333–338

    Google Scholar 

  • Wright JC, Lacy ML (1988) Increase of disease resistance in celery cultivars by regeneration of whole plants from cell suspension cultures. Plant Dis 72:256–259

    Google Scholar 

  • Zong-xiu S, Cheng-Zhang Z, Kang-le Z, Xiu-fang Q, Ya-Ping F (1983) Somaclonal genetics of rice Oryza sativa. Theor Appl Genet 17:67–73

    Google Scholar 

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Authors and Affiliations

  1. Department of Vegetable Crops, Plant Growth Laboratory, University of California, 95616, Davis, CA, USA

    S. Heath-Pagliuso & L. Rappaport

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  1. S. Heath-Pagliuso
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  2. L. Rappaport
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Communicated by A. L. Kahler

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Heath-Pagliuso, S., Rappaport, L. Somaclonal variant UC-T3: the expression of Fusarium wilt resistance in progeny arrays of celery, Apium graveolens L.. Theoret. Appl. Genetics 80, 390–394 (1990). https://doi.org/10.1007/BF00210078

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  • DOI: https://doi.org/10.1007/BF00210078

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