Summary
The effect of rat serum versus fetal calf serum on the in vitro natural cytolytic activity of rat lymphocytes, macrophages and polymorphonuclear cells against syngeneic tumour cells was compared. The cytolysis level mediated by the three varieties of effector cells was lower when rat serum was used instead of fetal calf serum to supplement the culture medium. This could explain in part the discrepancies found between in vitro and in vivo studies.
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Caignard A, Lagadec P, Reisser D, Jeannin JF, Martin MS, Martin F (1985) Role of macrophage in the defense against intestinal cancers. Comp Immunol Microbiol Infect Dis 8:147
Caignard A, Martin MS, Michel MF, Martin F (1985) Interaction between two cellular subpopulations of a rat colonic carcinoma when inoculated to the syngeneic host. Int J Cancer 36:273
Carvajal RE, Maldonado G (1986) Effect of serum on autologous cell recognition by macrophages. Cell Biol Int Rep 10:101
Fady C, Lagadec P, Pelletier H, Sauvageot F, Reisser D (1986) Activité tumoricide in vitro de macrophages péritonéaux et de lymphocytes spléniques de rats soumis à un régime avec ou sans poisson. CR Soc Biol (Paris) 180:426
Fogel M, Segal S, Gorelik E, Feldman M (1978) Specific cytotoxic lymphocytes against tumors are generated in culture in the presence of syngeneic, but not xenogeneic, serum. Int J Cancer 22:329
Forni G, Green I (1976) Heterologous sera: a target for in vitro cell mediated cytotoxicity. J Immunol 116:1561
Freshney RI (1983) Culture of animal cells. Alan R Liss, New York
Jeannin JF, Olsson NO, Martin F (1983) Liposome induction or enhancement of macrophage mediated cancer cell lysis. Int J Cancer 31:75
Lagadec P, Jeannin JF, Milan C, Reisser D (1985) Stain dependence of rat macrophage natural tumoricidal capacity and its stimulation by endotoxins. Immunology Lett 9:117
Martin F, Martin MS, Jeannin JF, Lagneau A (1978) Rat macrophage mediated toxicity to cancer cells: effect of endotoxins and endotoxin inhibitors contained in culture media. Eur J Immunol 8:607
Morrison DG, Jacobs DM (1976) Binding of polymyxin B to the lipid-A portion of bacterial lipopolysaccharides. Immunobiochemistry 13:813
Nakagawara M, Takeshige K, Taramatsu J, Takahashi S, Yoshitake J, Minakami S (1986) Inhibition of superoxide production and Ca2+ mobilization in human neutrophils by halothane, enflurane and isoflurane. Anesthesiology 64:4
Pelletier H, Olsson NO, Shimizu T, Lagadec P, Fady C, Reisser D, Jeannin JF (1987) In vitro natural killer activity against progressive and regressive variants of a rat colon adenocarcinoma. Effect of treatments with anti-asialo GM1 plus complement. Immunobiology 175:202
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Reisser, D., Fady, C., Pelletier, H. et al. Comparative effect of rat and fetal calf serum on measurement of the natural tumoricidal activity of rat lymphocytes, macrophages and polymorphonuclear cells. Cancer Immunol Immunother 28, 34–36 (1989). https://doi.org/10.1007/BF00205798
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DOI: https://doi.org/10.1007/BF00205798