Summary
Treatment in vitro of human peripheral blood lymphocytes (PBL) with ConA induced the generation of suppressor cells which inhibited T cell blastogenic response to ConA and of allogeneic response in the mixed lymphocyte reaction (MLR). Treatment of PBL with 4-hydroperoxycyclophosphamide (4-HPCy) before incubation with ConA markedly decreased the generation of suppressor cells by ConA. The effect of 4-HPCy on generation of suppressor cells was more pronounced in the test of ConA stimulation than in the MLR. Treatment with 4-HPCy had no effect on suppressor cells already induced as shown by incubation of PBL with 4-HPCy after incubation with ConA.
Similar content being viewed by others
References
Askenase PW, Haysten BJ, Gershon RK (1975) Augmentation of delayed type hypersensitivity by doses of cyclophosphamide which do not affect antibody responses. J Exp Med 141:697
Berd D, Danna V, Maguire H, Mastrangelo M (1982) Selective impairment of suppressor T cell function after administration of cyclophosphamide. Proc Am Assoc Cancer Res 23:279
Boyum A (1968) Separation of leukocytes from blood and bone marrow. Scand J Clin Lab Invest Suppl 21:97
Diamantstein T, Klos M, Hahn H, Kaufmann SHE (1981) Direct in vitro evidence for different susceptibilities to 4-hydroperoxycyclophosphamide of antigen-primed T cells regulating humoral and cell mediated immune responses to sheep erythrocytes. J Immunol 126:1717
Gill HK, Liew FY (1978) Regulation of delayed type hypersensitivity. Eur J Immunol 8:172
Hallgren HM, Yunis EJ (1977) Suppressor lymphocytes in young and aged humans. J Immunol 118:2004
Kaufmann SHE, Hahn H, Diamanstein T (1980) Relative susceptibility of T cell subsets involved in delayed-type hypersensitivity to sheep red blood cells to the in vitro action of 4-hydroperoxycyclophosphamide. J Immunol 125:1104
L'Age-Stehr J, Diamanstein T (1978) Induction of autoreactive T lymphocytes and their suppressor cells by cyclophosphamide. Nature 271:663
Maguire H, Berd D, Engstrom P, Manstrangelo M (1981) Cyclophosphamide increases the acquisition of T cell immunity in patients with melanoma and colo-rectal cancer. Proc Am Assoc Cancer Res 22:279
Ozer HJ, Cowens W, Colvin M, Blumenson A, Sheedy D (1982) In vitro effect of 4-hydroperoxycyclophosphamide on human immunoregulatory T subset function. J Exp Med 155:276
Shand FL (1979) Review/Commentary. The immunopharmacology of cyclophosphamide. J Immunopharmacol 1:165
Shand FL, Howard JG (1979) Induction in vitro of reversible immunosuppression and inhibition of B cell receptor regeneration by defined metabolites of cyclophosphamide. Eur J Immunol 9:17
Shou L, Schwartz SA, Good RA (1976) Suppressor cell activity after concanavalin A treatment of lymphocytes from normal donors. J Exp Med 143:1100
Winer BJ (1971) In: Winer BJ (ed) Statistical principles in experimental design, 2nd edn. McGraw-Hill, London New York
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Klajman, A., Drucker, I., Manor, Y. et al. In vitro effects of 4-hydroperoxycyclophosphamide on concanavalin A-induced human suppressor T cells. Cancer Immunol Immunother 18, 113–117 (1984). https://doi.org/10.1007/BF00205744
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00205744