Summary
The peripheral blood leukocytes from 29 patients with adenocarcinoma of the colon were studied sequentially for the T-cell level, the rosette-inhibition titer of antithymocyte globulin and the blastogenic response to PHA and Con A to evaluate the T-cell immunocompetence. The level of E-rosette-forming cells and the blastogenic response did not reflect the immunocompetence of the T-cell population. Fluctuations in the rosette-inhibition titer of antithymocyte globulin (ATG) were observed; an increased ATG titer indicated an unfavorable clinical course, while a decreased ATG titer was observed with patients who had a favorable clinical course. The rosette-inhibition titer with antithymocyte globulin was observed to be an important indicator of competent T cells in patients with colorectal cancer.
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Bewick, M., Ogg, C. S., Parsons, V., Snowdon, S. A., Manuel, L.: Further assessment of rosette-inhibition test in clinical organ transplantation. Br. Med. J. 3, 491 (1972)
Farid, N. R., Munro, R. E., Row, V. V., Volpe, R.: Rosette inhibition test for the demonstration of thymus-dependent lymphocyte sensitization in Graves' disease and Hashimoto's thyroiditis. N. Engl. J. Med. 289, 1111 (1973)
Gattringer, C., Wick, G.: Relationship between E receptors and a T-specific surface antigen on human T cells. Immunology 32, 199 (1977)
Gross, R. L., Latty, A., Williams, E. A., Newberne, P. M.: Abnormal spontaneous rosette formation and rosette inhibition in lung carcinoma. N. Engl. J. Med. 292, 439 (1975)
Gross, R. L., Levin, A. G., Steel, C. M., Singh, S., Brubaker, G., Peers, F. G.: In vitro immunological studies on East African cancer patients. II. Increased sensitivity of blood lymphocytes from untreated Burkitt lymphoma patients to inhibition of spontaneous rosette formation. Int. J. Cancer 15, 132 (1975)
Horowitz, S., Groshong, T., Albrecht, R., Hong, R.: The “active” rosette test in immunodeficiency diseases. Clin. Immunol. Immunopathol. 4, 405 (1975)
Morton, H., Rolfe, B., Clunie, G. J. A., Anderson, M. J., Morrison, J.: An early pregnancy factor detected in human serum by the rosette inhibition test. Lancet I, 394 (1977)
Munro, A., Bewick, M., Manuel, L., Cameron, J. S., Ellis, F. G., Boulton-Jones, M., Ogg, C. S.: Clinical evaluation of a rosette inhibition test in renal allotransplantation. Br. Med. J. 3, 271 (1971)
Oldham, R. K., Weese, J. L., Herberman, R. B., Perlin, E., Mills, M., Heims, W., Blom, J., Green, D., Reid, J., Bellinger, S., Law, I., McCoy, J. L., Dean, J. H., Cannon, G. B., Djeu, J.: Immunological monitoring and immunotherapy in carcinoma of the lung. Int. J. Cancer 18, 739 (1976)
Owen, F. L., Fanger, M. W.: Studies on the human T-lymphocyte population. II. The use of a T-cell specific antibody in the partial isolation and characterization of the human lymphocyte receptor for sheep red blood cells. J. Immunol. 113, 1138 (1974)
Pauly, J. L., Sokal, J. E., Han, T.: Whole-blood culture technique for functional studies of lymphocyte reactivity to mitogens, antigens, and homologous lymphocytes. J. Lab. Clin. Med. 82, 500 (1973)
Small, P., Kashiwagi, N., Kohler, P. F.: Studies of the sheep red blood cell receptor using anti-lymphocyte antibodies. Cell. Immunol. 25, 302 (1976)
Special Technical Report: Identification, enumeration, and isolation of B and T lymphocytes from human peripheral blood. Scand. J. Immunol. 3, 521 (1974)
Wenzel, K. L., Ichiki, A. T.: A subpopulation of T cells detected by AET-treated sheep erythrocytes. Clin. Immunol. Immunopathol. 8, 73 (1977)
Wybran, J., Fudenberg, H. H.: Thymus-derived rosette-forming cells. N. Engl. J. Med. 288, 1072 (1973)
Wybran, J., Fudenberg, H. H.: Thymus-derived rosette-forming cells in various human disease states: cancer, lymphoma, bacterial and viral infections, and other diseases. J. Clin. Invest. 52, 1026 (1973)
Wybran, J., Levin, A. S., Spitler, L. E., Fudenberg, H. H.: Rosette-forming cells, immunologic deficiency diseases and transfer factor. N. Engl. J. Med. 288, 710 (1973)
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Supported by the Physicians' Medical Education and Research Foundation and General Research Supports Funds
Abbreviations used that are not spelled out in the text are: AET 2-aminoethylisothiouronium bromide; ATG Antithymocyte globulin; ALG Antilymphocyte globulin; E-RFC Erythrocyte rosette-forming cells; PHA Phytohemagglutinin; Con A Concanavalin A; E Sheep erythrocyte
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Ichiki, A.T., Collmann, I.R., Sonoda, T. et al. Inhibition of rosette formation by antithymocyte globulin. Cancer Immunol Immunother 3, 119–124 (1977). https://doi.org/10.1007/BF00200069
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DOI: https://doi.org/10.1007/BF00200069