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Site-related differences in the localization of the monoclonal antibody OX7 in SL2 and SL1 lymphomas

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Summary

The uptake of a monoclonal antibody (OX7) by murine lymphomas (SL1, SL2) growing in two sites in the mouse were compared. SL2 tumors grown in the subrenal site showed greater specific antibody uptake than did the same tumor grown in the subcutaneous site. Major differences in membrane bound antibody, in vitro antibody binding patterns, and gamma scintillation camera imaging were also observed between the two sites. These differences may be due to the greater blood flow measured in tumors growing in the subrenal capsule than those growing at the subcutaneous site. The differences observed in antibody uptake of the same tumor growing in two different sites raises questions concerning the choice of animal model systems that can be used to predict clinical utility.

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References

  1. Accolla RS, Carrel S, Mach J-P (1980) Monoclonal antibodies specific for carcinoembroyonic antigen and produced by two hybrid cell lines. Proc Natl Acad Sci USA 77:563–566

    Google Scholar 

  2. Ackerman NB, Hechmer PA (1977) Comparison of vascular permeability of tumors and liver host tissues. Bibl Anat 15:304–306

    Google Scholar 

  3. Ballou B, Levine G, Hakala RJ, Solter D (1979) Tumor location detected with radioactively labelled monoclonal antibodies and external scintigraphy. Science 206:844–847

    Google Scholar 

  4. Bogden A, Kelton D, Cobb W, Esber H (1978) A rapid screening method for testing chemotherapeutic agents against human xenografts, In: Houchens DP, Ovejera AA, Fischer Gustav (eds) Proceedings of Symposium on the Use of Athymic (Nude) Mice in Cancer Research. New York, pp 231–250

  5. Colcher D, Keenan AM, Larson SM, Schlom J (1981) Prolonged binding of a radiolabeled monoclonal antibody (B72.3) used in the in situ radioimmunodetection of human colon carcinoma xenografts. Cancer Res 44:5744–5751

    Google Scholar 

  6. Colcher D, Zalutsky M, Kaplan W, Kufe D, Austin F, Schlom J (1983) Radiolocalization of human mammary tumors in athymic mice by a monoclonal antibody. Cancer Res 43:736–742

    Google Scholar 

  7. Gallagher BM (1983) Monoclonal antibodies. The design of appropriate cancer and evaluation systems. In: Lambrecht R, Eckelman WC (eds) Animal Models in Radiotracer Design. Springer-Verlag, New York, pp 66–105

    Google Scholar 

  8. Gullino PM, Grantham FH (1961) Studies on the exchange of fluids between host and tumor. II. The blood flow of hepatomas and other tumors in rats and mice. J Natl Cancer Inst 27:1465–1491

    Google Scholar 

  9. Haskell CM, Buchegger F, Schreyer M, Carrel S, Mach J-P (1983) Monoclonal antibodies to carcinoembryomic antigen: ionic strength as a factor in the selection of antibodies for immunoscintography. Cancer Res 43:3857–3864

    Google Scholar 

  10. Houston LL, Nowinski RC, Bernstein ID (1980) Specific localization of monoclonal antibodies directed against the Thy 1.1 antigen. J Immunol 125:837–843

    Google Scholar 

  11. Khazaeli MB, Grown LE, Kabaza GA, Sargent EW, Beierwaltes WH (1984) Selection of monoclonal antibodies to HCG which localizes in human choriocarcinoma growing in the Syrian hamster cheek pouch. Hybridoma 3:41–48

    Google Scholar 

  12. Kohler G, Milstein C (1975) Continuous culture of fused cell secreting antibody of predefined specificity. Nature 256:495–497

    CAS  PubMed  Google Scholar 

  13. Midoux P, Maillet T, Therain F, Monsigny M, Roche AC (1984) Tumor localization of Lewis lung carcinoma with radiolabeled monoclonal antibodies. Cancer Immunol Immunother 18:19–23

    Google Scholar 

  14. Moldofsky PJ, Powe J, Mulhern CB, Hammond N, Sears HF, Gatenby RA, Steplewski Z, Koprowski H (1983) Metastatic colon carcinoma, detected with radiolabeled F(ab')2 monoclonal antibody fragments. Radiology 149:549–555

    Google Scholar 

  15. Moshakis V, McIlhinney RAJ, Raghavan D, Neville AM (1981) Localization of human tumour xenografts after I. V. administration of radiolabelled monoclonal antibodies. Br J Cancer 44:91–99

    Google Scholar 

  16. Sands H, Gallagher BM, Jones PL, Vaccaro D, Camin LL (1982) Evaluation of the “Thy System” for exploring the potential of monoclonal antibodies for tumor imaging and drug delivery. Int J Immunopharmacol 4:29

    Google Scholar 

  17. Sands H, Jones PJ, Neacy W, Shah SA, Gallagher BM (1984) The imaging of small experimental murine tumors grown in the subrenal capsule using monoclonal antibodies. Cancer Lett 24:65–72

    Google Scholar 

  18. Sands H, Shah SA, Gallagher BM (1985) Vascular volume and permeability of human and murine tumors grown in Athymic mice. Cancer Lett 27:15–21

    Google Scholar 

  19. Scheinberg DA, Strand M, Gansow OA (1982) Tumor imaging with radioactive metal chelates conjugated to monoclonal antibodies. Science 215:1511–1513

    Google Scholar 

  20. Wahl RL, Parker CW, Philpott GW (1983) Improved radioimaging and tumor localization with monoclonal F(ab')2. J Nucl Med 24:316–325

    Google Scholar 

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Authors and Affiliations

  1. Biomedical Products Department, Immunopharmaceutical Research and Development Department, E. I. duPont de Nemours and Co. (Inc.), 331 Treble Cove Road, 01862, North Billerica, Massachusetts, USA

    Howard Sands, Peter L. Jones, William P. Neacy, Sudhir A. Shah & Brian M. Gallagher

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  1. Howard Sands
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  2. Peter L. Jones
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  3. William P. Neacy
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  4. Sudhir A. Shah
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  5. Brian M. Gallagher
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Sands, H., Jones, P.L., Neacy, W.P. et al. Site-related differences in the localization of the monoclonal antibody OX7 in SL2 and SL1 lymphomas. Cancer Immunol Immunother 22, 169–175 (1986). https://doi.org/10.1007/BF00200028

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  • DOI: https://doi.org/10.1007/BF00200028

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