Abstract
A xyloglucan oligosaccharide was isolated from cell walls of Daucus carota L. suspension-cultured cells. From analytical data (gel-permeation chromatography, thin-layer chromatography, monosaccharide analysis, methylation analysis) it can be concluded that this oligosaccharide preparation consists mainly of a nonasaccharide known as XG9 (Glc4Xyl3GalFuc). This nonasaccharide showed excellent “anti-auxin” properties in the pea-stem bioassay, with 80% inhibition of 2,4-dichlorophenoxyacetic acid (2,4-D)-induced longitudinal growth of etiolated pea stem segments at concentrations of 1-0.1 nM. Applied in nanomolar concentrations to protoplasts regenerating in a medium containing 4.52 μM 2,4-D, the nonasaccharide influenced the viability of the protoplasts and the activities of glycan synthases in vitro. The effects were similar to those achieved by the omission of 2,4-D from the regeneration medium. The composition of the regenerated cell wall was not changed significantly by the use of 2,4-D-depleted medium or the addition of XG9 to 2,4-D-containing medium.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- XG9:
-
xyloglucan nonasacharide (Glc4Xyl3GalFuc)
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The authors wish to thank R. Hofmann for excellent technical assistance and Dr. K. Poralla for enabling them to use the Shimadzu GC-9A. They also wish to express their gratitude to Dr. S.C. Fry, Edinburgh, for carefully reading the manuscript. M.E. thanks all the people of GP IV for valuable discussion. M.E. was supported by a grant (Graduierten-Förderung) of the Land Baden-Württemberg.
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Emmerling, M., Seitz, H.U. Influence of a specific xyloglucan-nonasaccharide derived from cell walls of suspension-cultured cells of Daucus carota L. on regenerating carrot protoplasts. Planta 182, 174–180 (1990). https://doi.org/10.1007/BF00197107
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DOI: https://doi.org/10.1007/BF00197107