Abstract
Introduction of foreign genes into plant tissues via Agrobacterium tumefaciens based vectors requires specific knowledge of Agrobacterium-host compatibility. Therefore, to develop a transformation protocol for peanut (Arachis hypogaea L.), five Brazilian cultivars were screened with four wild-type A.tumefaciens strains. Successful transformation was dependent on specific bacterial strain-plant cultivar interactions and strain A281 was the most effective for tumor induction. Tumors displayed hormone autonomous growth, were opine positive and contained DNA that was homologous to the T-DNA of the inciting strain. Tumors induced on seed and seedling explants by A281 (pTD02) also expressed the reporter genes gus and npt-II contained in the binary vector. These results show that peanut is a permissive host for the acceptance of genes from specific A.tumefaciens gene vectors.
Similar content being viewed by others
Abbreviations
- GUS:
-
ß-glucuronidase (EC 3.2.1.31)
- NPT-II:
-
neomycin phosphotransferase II (EC 2.7.1.95)
- EDTA:
-
ethylene-diamine-tetracetic acid
References
Alliotte T, Tiré C, Engler G, Peleman J, Caplan A, Montagu MV Inzé D. 1989. Plant Physiol., 89:743–752.
Byrne MC, McDonnell RE, Wright M, Carnes MG. 1987. Plant Cell, Tissue Organ Cult., 8:3–15.
Deak M, Kiss G, Koncz C, Dudits D. 1986. Plant Cell Rep., 5:97–100.
Dellaporta SL, Wood J, Hicks JB. 1983. Plant Mol. Biol. Rep., 1(4):19–21.
Ditta G, Stanfield S, Corbin D, Helinski DR. 1980. Proc. Natl. Acad. Sci. USA, 77:7347–7351.
Dong JD, Bi YP, Xia LS, Sun SM, Song ZH, Guo B, Jiang XC, Shao QQ. 1990. Acta Genet. Sin, 17(1):13–16.
Hinchee MAW, Connor-Ward DV, Newell CA, McDonnell RE, Sato SJ, Gasser CS, Fischhoff DA, Re DB, Fraley RT, Horsch RB. 1988. Bio/technology, 6:915–922.
Hood EE, Chilton M-D, Fraley RT. 1986a. J. Bacteriol. 168:1283–1290.
Hood EE, Fraley RT, Chilton M-D. 1987. Plant. Physiol. 83:529–534.
Hood EE, Helmer GL, Fraley RT, Chilton M-D. 1986b. J. Bacteriol., 168:1291–1301.
Jefferson RA, Kavanagh TA, Bevan MW. 1987. Embo J., 13:3901–3907.
Jin S, Komari T, Gordon M, Nester EW. 1987. J. Bacteriol. 169:4417–4425.
Manners JM. 1987. Plant Cell Rep., 6:204–207.
McDonnell RE, Clark RD, Smith WA, Hinchee MA. 1987. Plant Mol. Biol. Rep., 5:380–386.
McKently AH, Moore GA, Gardner FP. 1990. Crop. Sci., 30:192–196.
Mugnier J. 1988. Plant Cell Rep., 7:9–12.
Murashige T, Skoog F. 1962. Physiol. Plant., 15:473–497.
Otten LABM, Schilperoort RA. 1978. Biochem. Biophys. Acta, 527:497–500.
Owens LD, Cress DE. 1985. Plant Physiol., 77:87–94.
Puonti-Kaerlas J, Stabel P, Eriksson T. 1989. Plant Cell Rep., 8:321–324.
Reynaerts A, De Block M, Hernalsteens J-P, Van Montagu M. 1989. In: Gelvin SB, Schilperoort RA, (eds.). Plant Molecular Biology Manual, Kluwer Academic Publishers, The Netherlands., pp. A9 1–15.
Sciaky D, Montoya AL, Chilton M-D. 1978. Plasmid, 1:238–253.
Scott R. 1988. In: Draper J, Scott R, Armitage P, (eds.). Plant Genetic Transformation, A Laboratory Manual, Blackwell Scientific Publications, pp. 237–261.
Author information
Authors and Affiliations
Additional information
Communicated by I. K. Vasil
Rights and permissions
About this article
Cite this article
Lacorte, C., Mansur, E., Timmerman, B. et al. Gene transfer into peanut (Arachis hypogaea L.) by Agrobacterium tumefaciens . Plant Cell Reports 10, 354–357 (1991). https://doi.org/10.1007/BF00193158
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00193158