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Enzymatic synthesis of peptide in acetonitrile/supercritical carbon dioxide

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Summary

The peptide synthesis from N-acetyl-L-tyrosine ethyl ester (Ac-Tyr-OEt) and amino acid amides was realized using α-chymotrypsin (CT) in acetonitrile (MeCN) or acetonitrile/supercritical carbon dioxide (SCCO2) containing small amounts of water. In both solvent systems there was an optimum water content for peptide synthesis, above which peptide hydrolysis became more important. After an incubation for 5 hours, the yields of the peptide was 64% in MeCN and 91% in MeCN/SCCO2, respectively.

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Authors and Affiliations

  1. Department of Industrial Chemistry, Faculty of Technology, Tokyo Metropolitan University, Minami-Ohsawa, Hachioji, 192-03, Tokyo, Japan

    Hidetaka Noritomi, Motokazu Miyata, Satoru Kato & Kunio Nagahama

Authors
  1. Hidetaka Noritomi
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  2. Motokazu Miyata
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  3. Satoru Kato
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  4. Kunio Nagahama
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Noritomi, H., Miyata, M., Kato, S. et al. Enzymatic synthesis of peptide in acetonitrile/supercritical carbon dioxide. Biotechnol Lett 17, 1323–1328 (1995). https://doi.org/10.1007/BF00189219

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  • DOI: https://doi.org/10.1007/BF00189219

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