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Spontaneous phenotypic and karyotypic progression in the SV40 transfected cell line SVG during prolonged passage in vitro

  • Laboratory Investigation
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Summary

Transfection of primary cultures of human cells with origin of replication deficient SV40 DNA has been carried out by others to generate in vitro models of malignant transformation in vivo. The present work describes progressive alterations in karyotype and phenotype in one such transfected (neuroglial) cell line (SVG). After repeated passage, recognisable marker chromosomes evolved. These may be related to karyotypic anomalies found in human glial tumors. Accompanying the evolution in karyotype were changes in phenotype. Although presaging malignant transformation, these stopped short of actual tumorigenicity.

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Authors and Affiliations

  1. The Ontario Cancer Institute, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada

    P. Davey & A. Michael Rauth

  2. Department of Pathology, The Princess Margaret Hospital, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada

    L. Mason

  3. Department of Cytogenetics, The Wellesley Hospital, 160 Wellesley Street East, M4Y IJ3, Toronto, Ontario, Canada

    L. Addy

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  1. P. Davey
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  2. A. Michael Rauth
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  3. L. Mason
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  4. L. Addy
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Davey, P., Rauth, A.M., Mason, L. et al. Spontaneous phenotypic and karyotypic progression in the SV40 transfected cell line SVG during prolonged passage in vitro . J Neuro-Oncol 8, 13–22 (1990). https://doi.org/10.1007/BF00182082

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