Summary
Two strains (NCIB 11412 and NCIB 10814) of the thermophilic organism Bacillus stearothermophilus were found to produce complex carbohydrase systems. The enzyme activities in each system include α-amylase as the major component, maltase, pullulanase, a minor amylase and cyclodextrinase. The latter three activities are produced in low yield in both strains. A crude enzyme preparation from each strain possessed maltogenic properties on hydrolysis of soluble starch. Following rigorous purification procedures, the purified major α-amylase from either strain did not produce maltose as a major end-product of starch hydrolysis. However, a partially purified mixture of pullulanase, minor amylase and cyclodextrinase activities from NCIB 11412 and NCIB 10814 produced 56.4% and 62.0% maltose, respectively, from soluble starch.
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Fogarty, W.M., Brosnan, M.P., Doyle, E.M. et al. The carbohydrases of Bacillus stearothermophilus NCIB 11412 and NCIB 10814. Appl Microbiol Biotechnol 37, 191–196 (1992). https://doi.org/10.1007/BF00178169
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DOI: https://doi.org/10.1007/BF00178169