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Molecular analysis of the gene cluster involved in cephalosporin biosynthesis from Lysobacter lactamgenus YK90

  • Applied genetics and regulation
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Abstract

Determination of the nucleotide sequence downstream from the Lysobacter lactamgenus pcbC gene encoding isopenicillin N synthase revealed that five open-reading frames (ORF) including the pcbC gene were tightly linked in the same orientation. Each ORF has the remarkable feature of the protein-coding frame in the DNA sequence with a high G + C content. Expression in Escherichia coli and a comparison of the deduced amino acid sequences with published sequences showed that the gene cluster contained a deacetoxycephalosporin C synthetase (DAOCS) gene (cefE), an ORF having homology with the Cephalosporium acremonium DAOCS/deacetylcephalosporin C synthetase gene (cefEF), an isopenicillin N epimerase gene(cefD), and a β-lactamase gene. The gene order was pcbC-cefE-ORF3-cefD-β-lactamase.

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  1. H. Kimura, M. Izawa & Y. Sumino

    Present address: Biotechnology Department, Pharmaceutical Research Division, Takeda Chemical Industries Ltd., Yodogawa-ku, 532, Osaka, Japan

Authors and Affiliations

  1. Fermentation Center, Technology Development Laboratories, Takeda Chemical Industries Ltd., Yodogawa-ku, 532, Osaka, Japan

    H. Kimura, M. Izawa & Y. Sumino

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  1. H. Kimura
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  2. M. Izawa
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  3. Y. Sumino
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Kimura, H., Izawa, M. & Sumino, Y. Molecular analysis of the gene cluster involved in cephalosporin biosynthesis from Lysobacter lactamgenus YK90. Appl Microbiol Biotechnol 44, 589–596 (1996). https://doi.org/10.1007/BF00172490

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  • DOI: https://doi.org/10.1007/BF00172490

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