Skip to main content
SpringerLink
Log in

Production of \-mannanases by Trichoderma harzianum E58

  • Biotechnology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Summary

The extracellular mannanase and endoglucanase activities of Trichoderma harzianum E58 were followed during growth of the fungus on 1% (w/v) mannose, Avicel, locust bean gum, konjac powder or the water-soluble fraction from stream-treated white spruce (SWS). Peak galactomannanase activities of 0.60 IU/ml and 0.66 IU/ml were detected in culture filtrates after 6–8 days growth on locust bean gum and Avicel respectively. When SWS or konjac powder were used as substrates, lower but relatively constant levels of activity were detected between 2 and 11 days of growth. Growth of the fungus on mannan-rich locust bean gum resulted in the highest specific glucomannanase and galactomannanase values. Although growth on 1% mannose failed to induce any mannanase activity, when 0.5% galactomannan was added with mannose, mannanase activity was detected in the culture filtrate. This indicated that mannanase production was not repressed in the presence of mannose. Samples were taken from each culture at the time of maximum galactomannanase activity. A protein profile obtained by isoelectric focusing was followed by a zymogram overlay to detect bands exhibiting galactomannanase, glucomannanase and endoglucanase activities. Several bands showed mannanase and endoglucananase activity. One band at pI 6.55 revealed both gluco- and galactomannanase activity and was free of detectable cellulase activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Biely P (1985) Microbial xylanolytic systems. Trends Biotechnol 3:286–290

    Google Scholar 

  • Blum H, Beier H, Gross HJ (1987) Improved silver staining of plant proteins, RNA, DNA in polyacrylamide gels. Electrophoresis 8:93–99

    CAS  Google Scholar 

  • Bradford M (1976) A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Brownell HH, Saddler JN (1984) Steam explosion pretreatment for enzymatic hydrolysis. Biotechnol Bioeng Symp 14:55–68

    Google Scholar 

  • Clark TA, McDonald AG, Senior DJ, Mayers PR (1990) Mannanase and xylanase treatments of softwood chemical pulps: effects on pulp properties and bleachability. In: Kirk K, Chang HHM (eds) Biotechnology in Pulp and Paper Manufacture, Butterworth & Heinemann, Chap. 14, pp 153–167

  • Dekker RFH (1979) The hemicellulase group of enzymes. In: Blanshard JMV, Mitchell JR (eds) Polysaccharides in food. Butterworths, London, pp 93–108

    Google Scholar 

  • Dekker RFH, Richards GN (1976) Hemicellulases: their occurrence, purification, properties and mode of action. Adv Carbohydr Chem Biochem 32:277–352

    Google Scholar 

  • Emi S, Fukumoto J, Yamamoto T (1972) Crystallization and some properties of mannanase. Agric Biol Chem 36:991–1001

    CAS  Google Scholar 

  • Eriksson KE, Goodell EW (1974) Pleiotropic mutants of the wood-rotting fungus Polyporus adustus lacking cellulase, mannanase, and xylanase. Can J Microbiol 20:371–378

    Google Scholar 

  • Gherardini FC, Salyers AA (1987) Purification and characterization of a cell-associated, soluble mannanase from Bacteroides ovatus. J Bacteriol 169:2038–2043

    Google Scholar 

  • Ghose TK, Bisaria VS (1979) Studies on the mechanism of enzymatic hydrolysis of cellulosic substances. Biotechnol Bioeng 21:131–146

    Google Scholar 

  • Heukeshoven J, Dernick R (1985) Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining. Electrophoresis 6:103–112

    Google Scholar 

  • Hrmová M, Biely P, Vrsanska M (1986) Specificity of cellulase and β-xylanase induction in Trichoderma reesei QM 9414. Arch Microbiol 144:307–311

    Google Scholar 

  • Ishihara M, Shimizu K (1980) Hemicellulases of brown-rotting fungus Tyromyces palustris IV: purification and some properties of an extracellular mannanase. Mokuzai Gakkaishi 26:811–818

    Google Scholar 

  • Kantelinen A, Ratto M, Sundquist J, Rauna M, Viikari L, Linko M (1988) Hemicellulases and their potential role in bleaching. In: TAPPI International Pulp-Bleaching Conference, Orlando, Florida, USA, June, TAPPI Press, pp 1–13

  • Khan AW, Lamb KA, Johnson KG (1989) Formation of enzymes required for the hydrolysis of plant cell wall polysaccharides by Trichoderma reesei MIRCEN J 5:49–54

    Google Scholar 

  • Knapp JS, Legg M (1986) The effect of different cellulosic growth substrates and pH on the production of cellulolytic enzymes by Trichoderma reesei. J Appl Bacteriol 61:319–329

    Google Scholar 

  • Lyr H (1963) Über das Vorkommen von Mannanase bei Pilzen. Zeitschrift für Allg Mikrobiol 3:25–36

    Google Scholar 

  • Mandels M (1982) Cellulases. In: Tsao G (ed) Annual Report of the Fermentation Proceedings, Academic Press, vol 5, pp 35–78

  • Mandels M, Andreotti R, Roche C (1976) Measurement of saccharifying cellulose. Biotechnol Bioeng Symp 6:2–34

    Google Scholar 

  • McCleary BV (1979) Modes of action of β-mannanase enzymes of diverse origin on legume seed galactomannans. Phytochemistry 18:757–763

    Google Scholar 

  • McCleary BV (1988) β-d-Mannanases. Methods Enzymol 160:596–610

    Google Scholar 

  • Mes-Hartree M, Hogan CM, Saddler JN (1988) Influence of growth substrate on production of cellulase enzymes by Trichoderma harzianum E58. Biotechnol Bioeng 31:725–729

    Google Scholar 

  • Montenecourt BS, Eveleigh DE (1977) Preparation of mutants of Trichoderma reesei with enhanced cellulase production. Appl Environ Microbiol 34:777–784

    Google Scholar 

  • Paice MG, Bernier R, Jurasek L (1988) Viscosity-enhancing bleaching of hardwood kraft pulp with xylanase from a cloned gene. Biotechnol Bioeng 32:235–239

    Google Scholar 

  • Park GG, Kusakabe I, Komatsu Y, Kobayashi H, Yasui T, Murakami K (1987) Purification and some properties of β-mannanase from Penicillium purpurogenum. Agric Biol Chem 51:2709–2716

    Google Scholar 

  • Reese ET, Shibata Y (1965) β-Mannanases of fungi. Can J Microbiol 11:167–183

    CAS  PubMed  Google Scholar 

  • Saddler JN, Mes-Hartree M (1984) The enzymatic hydrolysis and fermentation of pretreated wood substrates. Biotechnol Adv 2:161–181

    Google Scholar 

  • Senior DJ, Mayers PR, Sutcliffe R, Tan L, Saddler JN (1988) Selective solubilization of xylan in pulp using a purified xylanases from Trichoderma harzianum. Biotechnol Lett 10:907–912

    Google Scholar 

  • Senior DJ, Mayers PR, Saddler JN (1989a) Production and purification of xylanases. In: Lewis NG, Paice MG (eds) Plant cell wall polymers: biogenesis and biodegradation (ACS Symposium Series no. 399). American Chemical Society, Toronto, Canada, pp 641–654

    Google Scholar 

  • Senior DJ, Mayers PR, Saddler JN (1989b) Xylanase production by Trichoderma harzianum E58. Appl Microbiol Biotechnol 32:137–142

    Google Scholar 

  • Sinner M, Parameswaran N, Dietrichs HH (1979) Degradation of delignified sprucewood by purified mannanase, xylanase and cellulases. Adv Chem Ser 181:303–329

    Google Scholar 

  • Somogyi M (1952) Notes on sugar determination. J Biol Chem 195:19–23

    CAS  PubMed  Google Scholar 

  • Takahashi R, Kusakabe I, Kobayashi H, Murakami K, Maekawa A, Suzuki T (1984) Purification and some properties of mannanase from Streptomyces sp. Agric Biol Chem 48:2189–2195

    Google Scholar 

  • Tan LL, Mayers PR, Illing M, Saddler JN (1987) The copurification of β-glucosidase, β-xylosidase and β-1,3-glucanase in the two separate enzyme complexes isolated from Trichoderma harzianum. Biochem Cell Biol 65:822–832

    Google Scholar 

  • TAPPI (1989) Test method T222 os-74, vol 1, Chemical Properties Committee Process and Product Development Division, Chairman, JC Abbott, TAPPI Press, Atlanta, Georgia, USA

    Google Scholar 

  • Urbanek H, Zalewsko-Sobezak J, Borowinska A (1978) Isolation and properties of extracellular cellulase-hemicellulase ocmplex of Phoma hibernica. Arch Microbiol 118:265–269

    Google Scholar 

  • Yamazaki VN, Sinner M, Dietrichs HH (1976) Isolierung und Eigenschaften einer β-1,4-Mannanase aus Aspergillus niger. Holzforschung 30:101–109

    Google Scholar 

  • Yu EKC, Tan LUL, Chan MKH, Deschatelets L, Saddler JN (1987) Production of thermostable xylanase by a thermophilic fungus Thermoascus aurantiacus. Enzyme Microbiol Technol 9:16–24

    Google Scholar 

Download references

Author information

Author notes

  1. J. P. Torrie, D. J. Senior & J. N. Saddler

    Present address: Department of Forest Products Biotechnology Faculty of Forestry, University of British Columbia, 2357 Main Mall, V6T 1W5, Vancouver, Canada

Authors and Affiliations

  1. Department of Biotechnology and Chemistry, Forintek Canada Corporation, 800 Montreal Rd, K1G 3Z5, Ottawa, Ontario, Canada

    J. P. Torrie, D. J. Senior & J. N. Saddler

Authors
  1. J. P. Torrie
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. J. Senior
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. N. Saddler
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Offprint requests to: J. N. Saddler

Rights and permissions

Reprints and permissions

About this article

Cite this article

Torrie, J.P., Senior, D.J. & Saddler, J.N. Production of \-mannanases by Trichoderma harzianum E58. Appl Microbiol Biotechnol 34, 303–307 (1990). https://doi.org/10.1007/BF00170047

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00170047

Keywords

Search

Navigation