Summary
In Gibberella fujikuroi, ammonium (NH4 +) interfered with the production of gibberellic acid (GA3). Optimal production occurred at 19 mm (NH4)2SO4 and the synthesis of GA3 was reduced threefold in a medium with 38 mm (NH4)2SO4. Using a resting cell system with mycelia previously grown on two concentrations (19 mm and 38 mm) of (NH4)2SO4, it was found that NH4 + depressed synthesis of the gibberellin-synthesizing enzymes. Furthermore, addition of NH4 + to a producing system shut off gibberellin formation, indicating that the negative effect of NH4 + ions is also due to inhibition of one or more enzymes in the gibberellin biosynthesis pathway. The onset of gibberellin biosynthesis in media with high (38 mm) and low (19 mm) concentrations of (NH4)2SO4 was studied by addition of cycloheximide to batch cultures of various ages.
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Brückner, B., Blechschmidt, D. Nitrogen regulation of gibberellin biosynthesis in Gibberella fujikuroi . Appl Microbiol Biotechnol 35, 646–650 (1991). https://doi.org/10.1007/BF00169631
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DOI: https://doi.org/10.1007/BF00169631