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Interactions between intracellular cyclic AMP and agonist-induced inositol phospholipid breakdown in isolated gastric mucosal cells of the rat

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Summary

The interactions between putative second effector mechanisms for hydrogen ion secretion were studied in isolated gastric cell preparations of the rat containing 60 - 70% parietal cells. Dibutyryl-CAMP and the compounds which increased the level of cAMP (histamine plus rolipram and forskolin plus rolipram) inhibited the carbachol-induced accumulation of [3H]inositol tris-, bis- and monophosphate. There was both a temporal and quantitative correlation between the increase in cAMP and the inhibition of the accumulation of [3H]inositol phosphates. Cimetidine attenuated the inhibitory effect of histamine on the formation of [3H]inositol phosphates. The enhancement of the accumulation of [3H]inositol phosphates by various concentrations of carbachol affected neither the basal nor the histamine-stimulated cAMP levels. In contrast to dibutyryl-cAMP, dibutyryl-cGMP did not modify the carbachol-induced formation of [3H]inositol phosphates. The biologically active phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), which activates protein kinase C, inhibited both the basal and carbachol-induced accumulation of [3H]inositol phosphates. We suggest that the inhibition of the formation of inositol trisphosphate by the increase in the intracellular level of cAMP and by the activation of protein kinase C might be intracellular negative feedback systems which prevent the overreaction of the acid-secreting parietal cells under the simultaneous influence of the physiological gastric secretagogues.

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Author notes

  1. Martin J. Lohse & Ulrich Schwabe

    Present address: Department of Pharmacology, University of Oulu, SF-90220, Oulu, Finland

Authors and Affiliations

  1. Pharmakologisches Institut der Universität, Im Neuenheimer Feld 366, D-6900, Heidelberg, Federal Republic of Germany

    Juhani Puurunen, Martin J. Lohse & Ulrich Schwabe

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  1. Juhani Puurunen
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  2. Martin J. Lohse
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  3. Ulrich Schwabe
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Fellow of the Alexander von Humboldt Foundation, FRG

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Puurunen, J., Lohse, M.J. & Schwabe, U. Interactions between intracellular cyclic AMP and agonist-induced inositol phospholipid breakdown in isolated gastric mucosal cells of the rat. Naunyn-Schmiedeberg's Arch Pharmacol 336, 471–477 (1987). https://doi.org/10.1007/BF00169301

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  • DOI: https://doi.org/10.1007/BF00169301

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