Summary
Citric acid production by immobilized of Aspergillus niger in a fluidized bed reactor was performed, evaluating the productivity and the stability of the process when pulsing device was used. The application of a pulsing flow to fluidized bed reactor and the feed nitrogen limited allow to control of bioparticles morphology avoiding bed compactation. When operated at optimum pulsation frequency (0.3 s−1) the stability of the bioreactor was maintained for more than 30 days, increasing the citric acid production in more than 52.2%.
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Pallares, J., Rodríguez, S. & Sanromán, A. Citric acid production by immobilized Aspergillus niger in a fluidized bed reactor. Biotechnol Tech 10, 53–58 (1996). https://doi.org/10.1007/BF00161084
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DOI: https://doi.org/10.1007/BF00161084