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Isolation of virally-infected insect cells from a population containing infected and uninfected cells

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Biotechnology Techniques

Abstract

A method was developed that can be used to isolate virally-infected insect cells from a mixed population containing infected and uninfected cells. Specifically, Spodoptera frugiperda Sf-9 cells infected with the Autographa californica multiple nuclear polyhedrosis virus were treated with a primary antibody specific for the gp64 protein present on the surface of virally-infected cells and a secondary antibody labeled with a fluorochrome. The resulting labeled cells were isolated by using fluorescence-activated cell sorting.

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Authors and Affiliations

  1. Department of Chemical and Biochemical Engineering, The University of Iowa, 52242-1219, Iowa City, Iowa, USA

    Alexander Matschiner, Jonathan S. Dordick & David W. Murhammer

Authors
  1. Alexander Matschiner
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  2. Jonathan S. Dordick
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  3. David W. Murhammer
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Matschiner, A., Dordick, J.S. & Murhammer, D.W. Isolation of virally-infected insect cells from a population containing infected and uninfected cells. Biotechnol Tech 9, 897–900 (1995). https://doi.org/10.1007/BF00158543

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  • DOI: https://doi.org/10.1007/BF00158543

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