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Effect of soybean oil on the enhanced expression of hirudin gene in Hansenula polymorpha

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Summary

A heterologous gene from bloodsucking leech for anticoagulant, hirudin, has been expressed in the methylotrophic yeast Hansenula polymorpha. The addition of 1%(v/v) soybean oil to the medium as a stabilizer enhanced the expression of the hirudin gene in H. polymorpha with MOX promoter. The production of hirudin in the medium with soybean oil was 320mg/L in a 5L fermenter.

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References

  • Andreaseen, A.A., and Stier, T.J.B. (1954). J. Cell. Comp. Physiol. 43, 271–282

    Google Scholar 

  • Bogdanova, A.I., Agaphonov, M.O., and Ter-Avanesyan, M.D.(1995). Yeast, 11, 343–353

    Google Scholar 

  • Brierley, R.A., Siegel, R.S., Bussineau, C.M., Craig, W.S., Holtz, G.C., Davis, G.R., and Buckholz, R.G. (1990). International Patent Application (PCT). WO 90/03431.

  • Buckholz, R.G., and Gleeson, M.A.G. (1991). Bio/Technology. 9, 1067–1072.

    Google Scholar 

  • Choi, E.S., Sohn, J.H., and Rhee, S.K. (1994). Appl. Microbiol. Biotechnol. 42, 587–594.

    Google Scholar 

  • Digan, M.E., Lair, S.V., Brierley, R.A., Siegel, R.S., Williams, M.E., Ellis, S.B., Kellaris, P.A., Provow, S.A., Craig, W.S., Velicelebi, G., Harpold, M.M., and Thill, G.P. (1989). Bio/Technology, 7, 160–164.

    Google Scholar 

  • van Dijken, J.P., Otto, R., and harder, W. (1976). Arch. Microbiol. 111, 137–144

    Google Scholar 

  • Gellissen, G., Janowicz, Z.A., Merckelbach, A., Piontek, M., Keup, P., Weydemann, U., Hollenberg, C.P., and Strasser, A.W.M. (1991). Bio/Technology, 9, 291–295.

    Google Scholar 

  • Janowicz, Z.A., Melber, K., Merckelbach, A., Jacobs, E., Harford, N., Comberbach, M., and Hollenberg, C.P. (1991). Yeast, 7, 431–443.

    Google Scholar 

  • Macreadie, I.G. (1989). Nucl. Acids Res. 18, 1078.

    Google Scholar 

  • de Roubin, M.R., Bastien, L., Shen, S.H., and Groleau, D. (1991). J. Industr. Microbiol. 8, 147–156.

    Google Scholar 

  • Sohn, J.H., Beburov, M.Y., Choi, E.S., and Rhee, S.K. (1993). J. Microbiol. Biotechnol. 3, 65–72.

    Google Scholar 

  • Sohn, J.H., Lee, S.K., and Choi, E.S., and Rhee, S.K. (1991). J. Microbiol. Biotechnol. 1, 266–273.

    Google Scholar 

  • Suutari, M., Liukkonen, K., and Laakso, S. (1990). J. Gen. Microbiol. 136, 1469–1474.

    Google Scholar 

  • Thomas, D.S., and Rose, A.H. (1979). Arch. Microbiol. 122, 49–55.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Applied Microbiology Research Division, Korea Research Institute of Bioscience and Biotechnology, KIST, P.O. Box 115, 305-600, Yusong, Taejon, Korea

    Chul-Ho Kim, Jung-Hoon Sohn, Eui-Sung Choi & Sang-Ki Rhee

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  1. Chul-Ho Kim
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  2. Jung-Hoon Sohn
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  3. Eui-Sung Choi
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  4. Sang-Ki Rhee
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Kim, CH., Sohn, JH., Choi, ES. et al. Effect of soybean oil on the enhanced expression of hirudin gene in Hansenula polymorpha . Biotechnol Lett 18, 417–422 (1996). https://doi.org/10.1007/BF00143463

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  • DOI: https://doi.org/10.1007/BF00143463

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