Summary
The inhibition of D-Amino Acid Oxidase by α-Keto Acid, product of the reaction with D-Amino Acid is described for the first time. Inhibition of the enzyme by α-Keto-γ-Methiolbutyric Acid (4-methylthio-2-oxobutanoic acid), product of the reaction with D-Methionine, was studied. From the results obtained it is deduced that inhibition is competitive with the substrate, the value of the inhibition constant being 1.85 10−3 M.
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Brachet, P., Carreira, S., Puigserver, A. (1990). Biochem. Int., 22, 837–842.
Brodelius, P., Nilsson, K., Mosbach, K. (1981). Applied Biochem. Biotechnol., 6, 293–308.
Cooper, A. J. L., Ginos, J. Z., Meister, A. (1983). Chem. Rev., 83, 321–358.
Deshpande, A., Sankaran, K., D'Souza, S. F., Nadkarni, G. B. (1987). Biotechnol. Techniques, 1, 55–58.
Dixon, M., Kleppe, K. (1965). Biochim. Biophys. Acta, 96, 357–367.
Dixon, M., Webb, E. C. (1979). Enzymes. 3rd, 349. New York: Academic Press.
Fink, D. J., Falb, R. D., Bean, M. (1978). AIChE Symp. Ser. 74, 18–24.
Fonda, M. L., Anderson, B. M. (1968). J. Biol. Chem., 243, 1931–1935.
Frisell, W. R., Lowe, H. J., Hellerman, L. (1956). J. Biol. Chem., 223, 75–83.
Galán, M. A. (1990). From Genes to Bioproducts. Proc 3rd Spanish Congress of Biotechnology, 319–323.
Guilbault, G. G., Hrabankova, P. (1971). Anal. Chim. Acta, 56, 285–290.
Hamilton, G. A., Buckthal, D. J. (1982). Bioorg. Chem., 11, 350–370.
Hamilton, G. A., Buckthal, D. J., Kalinyak, J. (1979). Oxidases Relat. Redox Syst., Proc. Int. Symp. 3rd, 447–459. Ed. King, T. E., Mason, H. S., Morrison, M. (1982), New York: Pergamon.
Klein, J. R., Austin, E. (1953). J. Biol. Chem. 205, 725–730.
Klein, J. R., Kamin, H. (1941). J. Biol. Chem. 138, 507–512.
Kubicek-Pranz, E. M., Röhr, M. (1985). Biotechnol. Lett., 7, 9–14.
Massey, V., Ganther, H. (1965). Biochemistry, 4, 1161–1173.
Szwajcer, D. E., Flygare, S., Mosbach, K. (1991). Applied Biochem. Biotechnol., 27, 239–250.
Szwajcer, D. E., Mosbach, K. (1985). Biotechnol. Lett., 7, 1–7.
Tosa, T., Sano, R., Chibata, I. (1974). Agr. Biol. Chem., 38, 1529–1534.
Tu, S., McCormick, D. B. (1972). Separation Sci. 7, 403–407.
Wikström, P., Szwajcer, D. E., Brodelius, P., Nilsson, K., Mosbach, K. (1982). Biotechnol. Lett., 4, 153–158.
Yagi, K., Ozawa, T., Okada, K. (1959). Biochim. Biophys. Acta, 35, 102–110.
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Moreno, J.A., Catalán, J. & Galán, M.A. Inhibition of D-amino Acid Oxidase by α-Keto-γ-Methiolbutyric Acid, product of the reaction with D-Methionine. Biotechnol Lett 16, 843–848 (1994). https://doi.org/10.1007/BF00133965
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DOI: https://doi.org/10.1007/BF00133965