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Overproduction of Serratia marcescens metalloprotease (SMP) from the recombinant Serratia marcescens strains

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Summary

To overproduce Serratia marcescens metalloprotease(SMP), various recombinant plasmids encoding SMP gene were constructed and the SMP productivities from the recombinant S. marcescens strains were examined. The recombinant S. marcescens strains indispensably required proteinaceous substrates such as casein for the extracellular production of SMP. We obtained maximum 9,100U/ml of SMP from the culture supernatant of S. marcescens ATCC27117 containing a regulatory plasmid pTSP2 encoding SMP gene fused with a strong trc99a promoter and its repressor gene lacIq, which is about 23 times higher than that of wild type strain. SMP produced from the recombinant S. marcescens(pTSP2) was 88.3% of total extracellular proteins.

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References

  • Amann, E., Ochs, B., and Abel, K.-J.(1988). Gene. 69. 301–315.

    Google Scholar 

  • Braun, V. and Schmilz, G.(1980). Arch. Microbiol. 124. 55–61.

    Google Scholar 

  • Braunagel, S. C. and Benedik, M. J.(1990). Mol. Gen. Genet. 222. 446–451.

    Google Scholar 

  • Bromke, B. J. and Hammel, J. M.(1979). Can. J. Microbiol. 25. 47–52.

    Google Scholar 

  • Eaves, G. and Jefferies, C. D.(1963). J. Bacteriol. 85. 273–278.

    Google Scholar 

  • Jung, G., Denefle, P., Becquart, J., and Mayaux, J.-F.(1988). Ann. Inst. Pasteur/Microbiol. 139. 129–146.

    Google Scholar 

  • Kim, K. S., Lee, C.-W., Lee, S. Y., Lee, B. R., and Shin, Y. C.(1992). Kor. J. Appl. Microbiol. Biotechnol. 20. 280–288.

    Google Scholar 

  • Kim, K. S., Kim, T. U., Kim, I. J., Byun, S. M., and Shin, Y. C. (1995). Appl. Environ. Microbiol., submitted.

  • Laemmli, U. K.(1970). Nature. 227.680–685.

    Google Scholar 

  • Miyazaki, H., Yanagida, N., Horinouchi, S., and Beppu, T.(1989). J. Bacteriol. 171. 6566–6572.

    Google Scholar 

  • Reid, J. D., Stoufer, S. D., and Ogrydziak, D. M.(1982). Gene. 17. 107–112.

    Google Scholar 

  • Sambrook, J., Fritsch, E. F., and Maniatis, T.(1989). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.

  • Shimizu, N., Fukuzono, S., Nishimura, N., Odawara, Y., and Fujiwara, K.(1987). J. Ferment. Technol. 65. 7–10.

    Google Scholar 

  • Tsai, L. B., Mann, M., Morris, F., Rotger, C., and Fenton, D.(1987). J. Ind. Microbiol. 2. 181–187.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Microbiology, College of Natural Sciences, Gyeongsang National University, 660-701, Chinju, Republic of Korea

    Ki Seok Kim, Koon Sig Park, Si Myung Byun, Jae Gu Pan & Yong Chul Shin

  2. Department of Life Science, Korea Advanced Institute of Science and Technology(KAIST), 305-701, Taejeon, Republic of Korea

    Si Myung Byun

  3. Biochemical Process Laboratory, Genetic Engineering Research Institute, KIST, 305-606, Taejon, Republic of Korea

    Jae Gu Pan

Authors
  1. Ki Seok Kim
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  2. Koon Sig Park
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  3. Si Myung Byun
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  4. Jae Gu Pan
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  5. Yong Chul Shin
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Kim, K.S., Park, K.S., Byun, S.M. et al. Overproduction of Serratia marcescens metalloprotease (SMP) from the recombinant Serratia marcescens strains. Biotechnol Lett 17, 497–502 (1995). https://doi.org/10.1007/BF00132017

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  • DOI: https://doi.org/10.1007/BF00132017

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