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Medium optimization for recombinant protein production by Bacillus subtilis

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Summary

Optimal concentrations of glucose, yeast extract and nutrient broth for the production of β-lactamase by B. subtilis were predicted using a statistical experimental design and then tested. More yeast extract (13 vs. 1 g/L), less glucose (7.4 vs. 10 g/L), and less nutrient broth (12.6 vs. 15 g/L) were required to achieve high β-lactamase activities (5700 U/L) instead of high cell growth rates (1.2 h-1).

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Author notes

  1. Kyungmoon Park

    Present address: Advanced Research Laboratory, Hitachi, Ltd., Hatoyama, 350-03, Saitama, Japan

Authors and Affiliations

  1. Department of Chemical and Bioresource Engineering, Colorado State University, 80523-1370, Fort Collins, CO, USA

    Kyungmoon Park & Kenneth F. Reardon

Authors
  1. Kyungmoon Park
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  2. Kenneth F. Reardon
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Park, K., Reardon, K.F. Medium optimization for recombinant protein production by Bacillus subtilis . Biotechnol Lett 18, 737–740 (1996). https://doi.org/10.1007/BF00130775

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  • DOI: https://doi.org/10.1007/BF00130775

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