The addition of concentrated nutrient solutions in fed-batch cultures of recombinant GS-NSO cells delays the onset of apoptosis by 24 to 48 hours and decreases the specific rate of apoptotic death approximately two-fold. This decrease in apoptosis appears primarily responsible for the increase in culture longevity that results in five- to ten-fold increases in final product concentrations in fed-batch cultures.
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DiStefano, D.J., Mark, G.E. & Robinson, D.K. Feeding of nutrients delays apoptotic death in fed-batch cultures of recombinant NSO myeloma cells. Biotechnol Lett 18, 1067–1072 (1996). https://doi.org/10.1007/BF00129733
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DOI: https://doi.org/10.1007/BF00129733