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A new system for the expression of recombinant antibody in mammalian cells

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Summary

New type of plasmids named pEdHCG1 and pEdHCκ.were constructed. A combination of two plasmids containing the amplified gene product for the variable region of the antibody (Ab) heavy or light chains by the polymerase chain reaction (PCR) was co-transfected into COS cells to transiently express and in Chinese hamster ovary (CHO) cells to constitutively express chimeric Ab composed of the mouse-derived variable region and human-derived constant region.

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Authors and Affiliations

  1. Tokyo Research Center, Tosoh Corporation, 2743-1, Hayakawa, Ayase-shi, 252, Kanagawa, Japan

    Yoshitaka Iba, Takashi Kaneko, Teiji Ekida, Kenji Miyata & Kiyoshi Yasukawa

  2. Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Sakyo-ku, 606-01, Kyoto, Japan

    Kuniyo Inouye

  3. Institute for Conprehensive Medical Science, Fujita Health University, Toyoake, 470-11, Aichi, Japan

    Yoshitaka Iba & Yoshikazu Kurosawa

Authors
  1. Yoshitaka Iba
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  2. Takashi Kaneko
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  3. Teiji Ekida
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  4. Kenji Miyata
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  5. Kuniyo Inouye
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  6. Yoshikazu Kurosawa
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  7. Kiyoshi Yasukawa
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Iba, Y., Kaneko, T., Ekida, T. et al. A new system for the expression of recombinant antibody in mammalian cells. Biotechnol Lett 17, 135–138 (1995). https://doi.org/10.1007/BF00127976

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  • DOI: https://doi.org/10.1007/BF00127976

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