Abstract
We have compared the temporal order of completion of replication of specific sites of X and 2nd chromosomes in two polytene cell types of D. hydei by examining the patterns of autoradiographic labelling in 3H-thymidine pulse (10 min) labelled salivary glands and gastric ceaca of mid 3rd instar larvae. Present results are in agreement with our earlier finding in D. nasuta (Lakhotia & Tiwari, 1984, Chromosoma, 89: 212–217 that in spites of a general similarity in the cytological identity of independently replicating sites in the two polytene cell types, their temporal programme of replication varies in different tissues. This may be related to differential gene activity patterns and polytene organization in the different cell types.
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References
Beermann, W., 1962. Riesenchromosomen. Protoplasmatologia Handbuch der Protoplasmaforschung. Band IVd (Springer-Verlag, Vienna)
Berendes H. D., The salivary gland chromosome of Drosophila hydei Sturtevant. Chromosoma 14: 195–206.
Berendes, H. D. & Meyer, G. F., 1968. A specific chromosome element, the telomere of Drosophila polytene chromosomes. Chromosoma 25: 184–197.
Evgen'ev, M., Levin, A. & Gubenko, I., 1977. Are late replicating regions in polytene chromosomes of Drosophila enriched by repeated nucleotide sequences?. Nature 268: 766–767.
Laird, C. D., 1980. Structural paradox of polytene chromosomes. Cell 22: 869–874.
Lakhotia, S. C. & Sinha, P., 1983. Replication in Drosophila chromosomes X. Two kinds of active replicons in salivary gland polytene nuclei and their relation to chromosomal replication patterns. Chromosoma 88: 265–276.
Lakhotia, S. C. & Tiwari, P. K., 1984. Replication in Drosophila chromosomes XI. Differences in temporal order of replications in two polytene cell types in D. nasuta. Chromosoma 89: 212–217.
Mishra, A. & Lakhotia, S. C., 1982. Replication in Drosophila chromosomes. VIII. Temporal order of replication of specific sites in polytene chromosomes from 24°C and 10°C reared larvae of Drosophila melanogaster. Ind. J. exp. Biol. 20: 652–659.
Redfern, C. P. F., 1981. DNA replication in polytene chromosomes: similarity of termination patterns in somatic and germ-line derived polytene chromosomes of Anopheles stephensi Liston (Diptera: Culicidae). Chromosoma 84: 33–47.
Richards, G., 1980. The polytene chromosomes in fat body nuclei of Drosophila melanogaster. Chromosoma 79: 241–250.
Rodman, T. C., 1968. Relationship of developmental stage to initiation of replication in polytene nuclei. Chromosoma 23: 271–287.
Roy, S. & Lakhotia, S. C., 1981. Replications in Drosophila chromosomes. Part IV. Patterns of chromosomal replication in salivary gland polytene nuclei of Drosophila nasuta. Ind. J. exp. Biol. 19: 797–807.
Rudkin, G. T., 1972. Replication in polytene chromosomes. In: Developmental studies in glant chromosomes (W.Beermann, ed.). Berlin, Heidelberg, New York-Spring: pp. 59–85.
Sinha, P. & Lakhotia, S. C., 1980. Replication in Drosophila chromosomes. Part V. Polytene chromosome replication after in vitro culture of larval salivary glands. Ind. J. exp. Biol. 18: 1059–1065.
Zhimulev, I. F., Semeshin, V. F., Kulichkov, V. A. & Belyaeva, E. S., 1982. Intercalary heterochromatin in Drosophila. I. Localization and general characteristics. Chromosoma 87: 197–228.
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Tiwari, P.K., Lakhotia, S.C. Replication in Drosophila chromosomes XIII. Comparison of late replicating sites in two polytene cell types in D. hydei . Genetica 65, 227–234 (1984). https://doi.org/10.1007/BF00122909
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DOI: https://doi.org/10.1007/BF00122909