Abstract
The karyotype of moose (2n=68) is characterized by very large C-bands close to the centromeres of most chromosomes. The C-banded material represents 40% of the genome. For further characterization of the heterochromatin chromosome spreads were treated with restriction endonucleases and the restriction enzyme (Re) banding pattern was analysed. HaeIII, AluI, MboI, RsaI and HinfI produced informative Re-bands. DdeI induced an even digestion with no banding. Staining with chromomycin A3 produced bright fluorescence in regions corresponding to C-bands. Labeling with BrdUrd during late S phase differentiates four regions in the C banded area. The sequence of these regions from centromere to telomere are: late, early, late and early replicating.
The authors propose the existence of five satellite DNA families with distinctive characteristics of G-C and A-T richness and different replication timing, and point out the different clusters for the endonucleases detailed above and their varying location in the chromosomes examined.
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Benedeum, U. M., Neitzel, H., Sperling, K., Bogenberger, J. & Fittler, F., 1986. Organization and chromosomal distribution of a novel repetitive DNA component from Muntiacus muntjak vaginalis with a repeat length of more than 40 kb. Chromosoma 94: 267–272.
Bianchi, M. S., Bianchi, N. O., Pantelias, G. E. & Wolff, S., 1985b. The mechanism and pattern of banding induced by restriction endonucleases in human chromosomes. Chromosoma 91: 131–136.
Bianchi, N. O. & Bianchi, M. S., 1987. Analysis of the eukaryotic chromosome organization with restriction endonucleases. In: G. Obe & A. Basler (eds), Cytogenetics, basic and applied aspects. Springer, Berlin, pp. 280–299.
Bianchi, N. O., Bianchi, M. S. & Cleaver, J. E., 1984. Relationship of ultraviolet light-induced DNA-protein crosslinkage to chromatin structure. Expl Cell Res. 156: 405–418.
Bianchi, N. O., Bianchi, M. S., Cleaver, J. E. & Wolff, S., 1985a. The pattern of restriction enzyme-induced banding in the chromosomes of chimpanzee, gorilla and orangutan and its evolutionary significance. J. mol. Evol. 22: 323–333.
Bogenberger, J., Neumaier, P. S. & Fittler, F., 1985. The muntjac satellite IA sequence is composed of 31-bp-pair internal repeats that are highly homologous to the 31-bp-pair subrepeats of the bovinesatellite 1.715. Eur. J. Biochem. 148: 121–144.
Catalá, A., Vidal-Rioja, L. & Bianchi, N. O., 1981. Liver chromatin fractions in Mus and Akodon. The concept of constitutive heterochromatin. Mol. Cell. Biochem. 36: 135–141.
Gripenberg, U., 1984. Characterization of the karyotype of the reindeer (Rangifer tarandus): the distribution of heterochromatin in the reindeer and the Scandinavian moose (Alces alces). 6th. Eur. Colloq. Cytogenet. Domest. Anim.: 68–79.
Gripenberg, U., Nygrén, T., Wessman, M. & Huuhtanen, S., 1986. The Finnish moose population; distinctive chromosome features. Suomen Riista 33: 59–66.
John, B., 1988. The biology of heterochromatin. In: R. Verma (ed.) Heterochromatin, molecular and structural aspects. Cambridge Univ. Press: 1–147.
Miller, D. A., Choi, Y. C. & Miller, O. J., 1983. Chromosome localization of highly repetitive human DNAs and amplified ribosomal DNA with restriction enzymes. Science 219: 395–397.
Neitzel, H., 1987. Chromosome evolution of Cervidae: karyotypic and molecular aspects. In: G. Obe & A. Basler (eds), Cytogenetics, basic and applied aspects. Springer, Berlin, pp. 90–112.
Perry, P. & Wolff, S., 1974. New Giemsa method for the differential staining of sister chromatids. Nature 251: 156–158.
Schweizer, D., 1976. Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma 58: 307–324.
Schweizer, D., 1977. R-banding produced DNase digestion of chromomycin-stained chromosomes. Chromosoma 65: 117–124.
Schweizer, D., 1980, Simultaneous fluorescent staining of R bands and specific heterochromatic regions (DA-DAPI bands) in human chromosomes. Cytogenet. Cell Genet. 27: 190–193.
Seuanez, H. N., 1979. The phylogeny of human chromosome. Springer Berlin.
Sumner, A. T., Evans, H. J. & Buckland, R. A., 1971. New technique for distinguishing between human chromosomes. Nature New Biol. 232: 31–32.
Sumner, A. T., 1972. A simple technique for demonstrating centromeric heterochromatin. Exp Cell RRes. 75: 304–306.
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Bianchi, M.S., Bianchi, N.O., Gripenberg, U. et al. Characterization of the heterochromatin in moose (Alces alces) chromosomes. Genetica 80, 1–7 (1990). https://doi.org/10.1007/BF00120114
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DOI: https://doi.org/10.1007/BF00120114