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Plant regeneration from leaf protoplasts of apple

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Abstract

Protoplasts were isolated from young leaves or etiolated shoot apices. For initiation of divisions the protoplasts were embedded in sodium alginate and cultivated in MS or MI medium supplemented with 2.2 μM BA, 2.6 μM NAA and 2.2 μM 2,4-dichlorophenoxyacetic acid. The protoplasts of all seven lines tested developed to protocalluses at high frequencies. No genotypic differences were observed. When BA was used in combination with NAA in the regeneration experiments, only a few protocalluses (highest frequency 3%) exhibited shoot organogenesis. When BA was replaced with thidiazuron, the percentage of protocalluses that developed shoots increased in two of three tested lines to 7% and 56%, respectively. Shoot development was achieved under light conditions. The shoots were then rooted and transferred into soil.

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Abbreviations

ABA:

abscisic acid

BA:

6-benzyladenine

2,4-d :

2,4-dichlorophenoxyacetic acid

FW:

fresh weight

GA3 :

gibberellic acid

IBA:

indole-3-butyric acid

MES:

2-N-morpholinoethane sulphonic acid

NAA:

α-naphthaleneacetic acid

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Perales, E.H., Schieder, O. Plant regeneration from leaf protoplasts of apple. Plant Cell Tiss Organ Cult 34, 71–76 (1993). https://doi.org/10.1007/BF00048465

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