Abstract
Multiple vegetative shoots and flowers emerged from the nodes of explants derived from young seedlings of the bamboo Dendrocalamus hamiltonii when cultured on a Murashige & Skoog based medium supplemented with the cytokinin 6-benzyladenine (BA). The largest number of shoots produced from intact cultured epicotyl tissue (ca 8 per explant) were obtained after 12 weeks on a medium containing 4.4 μM BA. The first flowers were observed in vitro after only 13 weeks from initial germination of the seedlings. The highest frequency of flowering (47% of nodal explants) was obtained when cultured explants were transferred from 22.2 μM BA to a growth regulator-free medium after 8 weeks.
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Abbreviations
- BA:
-
6-benzyladenine
- MS:
-
Murashige & Skoog
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Chambers, S.M., Heuch, J.H.R. & Pirrle, A. Micropropagation and in vitro flowering of the bamboo Dendrocalamus hamiltonii Munro. Plant Cell Tiss Organ Cult 27, 45–48 (1991). https://doi.org/10.1007/BF00048205
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DOI: https://doi.org/10.1007/BF00048205