Abstract
In vivo effect of abscisic acid (ABA) on photosynthetic oxygen evolution was investigated in barley chloroplasts. The most important kinetic parameters of O2-producing reactions were changed. The results show inhibition of the O2-flash yields at ABA concentrations of 10 μmol/l and 100 μmol/l and an increase in the degree of damping of the oscillations. ABA has a marked effect on the distribution of the oxygenevolving centers in S0 and S1 states and on sum of the centers (S0+S1) estimated according to the Kok model. In addition, the amplitude and the shape of the initial oxygen burst under continuous illumination are also significantly altered. At a concentration of 100 μmol/l, ABA strongly inhibits Hill reaction activity measured by DCPIP reduction. The results cannot be explained by the hypothesis of socalled “stomata effect”. On the other hand, no effects were observed on the investigated parameters in experiments involving ABA applied in vitro to isolated chloroplasts. It is hypothesized that ABA disrupts the granal chloroplasts structure and raises the degree of participation of the cooperative mechanism of O2-evolution connected with the functioning of PS IIβ centers in the stroma situated thylakoids.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- DCPIP:
-
2,6-Dichlorophenolindophenol
- DCMU:
-
3-(3,4-dichlorophenil)-1,1-dimethylurea
- HEPES:
-
N-2-Hydroxyethylpiperazine-N-2-ethane sulfonic acid
- PSII:
-
photosystem II
- RubisCO:
-
Ribulose-1,5-bis-phosphate carboxylase-oxygenase
References
Anderson JM and Melis A (1983) Localization of different photosystems in separate regions of chloroplast membranes. Proc Natl Acad Sci USA 80: 745–749
Bauer B, Huber W and Sankhla N (1976) Effect of abscisic acid on photosynthesis in Lemna minor L. Z Pflanzenphysiol 77: 237–246
Beevers Z, Lovey B, Pearson IA and Wareing PE (1970) Phytochrome and hormonal control of expansion and greening of etiolate wheat leaves. Planta (Berl) 90: 286–294
Bengtson C, Klockware B, Larsson C and Sundqist C (1977) The effect of phytohormones on the chlorophyll (ide), protochlorophyll (ide) and carotenoid farmation in greening dark grown wheat leaves. Physiol Plant 40: 198–204
Camm EL and Green BR (1980) Fractionation of thylakoid membranes with the nonionic detergent octyl-β-D-glucopyranoside. Plant Physiol 66: 428–432
Colquhoun A, Hillman J, Crewe C and Bowes B (1975) An ultrastructural study of the effects of abscisic acid on senescence of leaves of radish (Raphanus sativus L.). Protoplasma 84: 205–221
Dubbe DR, Farquhar CD and Raschke K (1978) Effect of abscisic acid on the grain of feedback loop involving carbon dioxide and stomata. Plant Physiol 62: 413–417
Heilmann B, Hartung W and Gimmler H (1980) The distribution of abscisic acid between chloroplasts and cytoplasm of leaf cells and permeability of the chloroplast envelope for abscisic acid. Z Pflanzenphysiol 97: 67–78
Hodgest M and Barber J (1983) The significance of the kinetic analysis of fluorescence induction in DCMU-inhibited chloroplasts in terms of photosystem 2 connectivity and heterogeneity. FEBS Letts 160: 177–181
Huber W and Sankhla N (1977) Studies on the influence of abscisic acid and gibberellic acid on the activities of some enzymes of carbohydrate metabolism in leaves of Pennisetum typhoides seedlings. Planta 116: 55–67
Joliot P and Joliot A (1968) A polarographic method for detection of oxygen production and reduction of Hill reagent by isolated chloroplasts. Biochim Biophys Acta 153: 625–634
Khokhlova VA, Karavaiko NN, Podergina TA and Kulaeva ON (1978) The antagonistic effect of abscisic acid and cytokinin on the structural and biochemical differentiation of chloroplasts in isolated pumpkin cotyledons (in Russian) Cytologia 20: 1033–1038
Kok B, Forbush B and McGloin M (1970) Cooperation of charges in photosynthetic O2 evolution. I. A linear four step mechanism. Photochem Photobiol 11: 457–475
Kunina M, Nikulovich TP and Kulaeva OH (1985) Coaction of abscisic acid and cytokinin in controlling the synthesis of plastid and cytoplasm ribosomal RNA in excised pumpkin cotyledons (in Russian). Physiol Rastenii 32: 298–309
Lehoczki E and Zeinalov Yu (1984) Unusual photosynthetic oxygen evolution I. Cerulenin-induced 3-(3,4-dichlorophenil)-1,1-dimethylurea insensitive oxygen evolution in Chlorella pyrenoidosa. Photobiochem Photobiophys 7: 135–142
Melis A (1985) Functional properties of photosystem II in spinach chloroplasts. Biochim Biophys Acta 808: 334–342
Melis A and Anderson JM (1983) Structural and functional organization of the photosystems in spinach chloroplasts. Antenna size, relative electron-transport capacity and chlorophyll composition. Biochim Biophys Acta 724: 473–484
Popova LP, Dimitrova OD and Vaklinova SG (1982) Effect of ABA on the chlorophyll content, the intensity of the photosynthetic CO2 fixation and the activity of the carboxylating enzymes in C3 and C4 plants. Compt Rend Acad Bulg Sci 35: 1291–1294
Popova LP, Tsonev DT and Vaklinova SG (1987) A possible role for abscisic acid in regulation of photosynthetic and photorespiratory carbon metabolism in barley leaves. Plant Physiol 83: 820–824
Raschke K and Hedrich R (1985) Simultaneous and independent effects of abscisic acid on stomata and photosynthetic apparatus in whole leaves. Planta 163: 105–118
Sankhla N and Huber W (1974) Enzyme activities in Pennisetum seedlings germinated in the presence of abscisic acid and gibberellic acid. Phytochemistry 13: 543–546
Schmid GH and Thibault P (1979) Characterization of a light-induced oxygen-uptake in tobacco protoplasts. Z Naturforsch 34: 570–575
Schreiber U and Pfister K (1982) Kinetic analysis of the light-induced chlorophyll fluorescence rise curve in the presence of dichlorophenyldimethylurea. Dependence of the slow-rise component on the degree of chloroplast intactness. Biochim Biophys Acta 680: 60–68
Velthuys B and Kok B (1978) Photosynthetic oxygen evolution from hydrogen peroxyde. Biochim Biophys Acta 502: 211–221
Wellburn FAU, Wellburn AR, Stoddart JL and Treharne KJ (1973) Influence of gibberellic and abscisic acids and the growth retardant, CCC, upon plastid development. Planta (Berl) 111: 337–346
Zanev Y and Maslenkova L (1987) Kinetics of oxygen-producting reactions and the effect of 3-(3,4-dichlorophenyl)-1,1-diemthylurea in relation with chloroplast structure. Fiziologia na rasteniata (Sofia) 1: 3–13 (Abstract in English)
Zeinalov Yu (1982) Existence of two different ways for oxygen evolution in photosynthesis and photosynthetic unit concept. Photosynthetica 16: 27–35
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Maslenkova, L.T., Zanev, Y. & Popova, L.P. Effect of abscisic acid on the photosynthetic oxygen evolution in barley chloroplasts. Photosynth Res 21, 45–50 (1989). https://doi.org/10.1007/BF00047174
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00047174