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Large scale fermentation and alkaloid production of cell suspension cultures of Catharanthus roseus

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Abstract

Cell cultures of Catharanthus roseus were scaled up to volumes of 50001 using conventional reactors equipped with flat-blade impellers. The behavior of the fermenter grown cells was compared with corresponding shake flask experiments with respect to growth and indole alkaloid inducibility and production. The limits and problems of transferring shake flask experiments of culture systems such as Catharanthus, in which alkaloid production depends greatly upon the physiological state of the cells, to large scale multistage processes is discussed.

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Authors and Affiliations

  1. GBF-Gesellschaft f. Biotechnologische Forschung m.b.H., Mascheroder Weg 1, D-3300, Braunschweig, BRD

    Otmar Schiel & Jochen Berlin

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  1. Otmar Schiel
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  2. Jochen Berlin
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Schiel, O., Berlin, J. Large scale fermentation and alkaloid production of cell suspension cultures of Catharanthus roseus . Plant Cell Tiss Organ Cult 8, 153–161 (1987). https://doi.org/10.1007/BF00043152

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