Summary
Plants were regenerated from callus cultures initiated from immature embryos of barley, Hordeum vulgare L. Immature embryos from seven diverse genotypes were cultured on modified Murashige and Skoog (MS) medium supplemented with 1.5 mg 2,4-D and 6.5 mg IAA/l. Of the 249 embryos cultured, 30% initiated callus within 8 days. Subculture of callus for 80 to 100 days on half-MS medium supplemented with 0.5 mg/l 2,4-D and 1.0 mg/l zeatin resulted in organogenesis. Culture of organogenic calli for 30 days on half-MS medium without growth regulators produced plants which originated mostly via multiple shoot formation. Callusing response of the tested genotypes ranged from zero to 44%; however, only 23% of the calli were regenerative. Regenerated plants included variants for chlorophyll deficiency, plant height, stem thickness, spike shape, pollen fertility, seed set and ploidy.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cheng, T. Y. & H. H. Smith, 1975. Organogenesis from callus culture of Hordeum vulgare. Planta (Berl.) 123: 307–310.
Dale, P. J. & E. Deambrogio, 1979. A comparison of callus induction and plant regeneration from different explants of Hordeum vulgare. Z. Pflanzenphysiol. 94: 65–77.
Foroughi-Wehr, B., W. Friedt & G. Wenzel, 1982. On the genetic improvement of androgenetic haploid formation in Hordeum vulgare L. Theor. Appl. Genet. 62: 233–239.
Goldstein, C. S. & W. E. Kronstad, 1986. Tissue culture and plant regeneration from immature embryo explants of barley, Hordeum vulgare. Theor. Appl. Genet. 71: 631–636.
Hanzel, J. J., J. P. Miller, M. A. Brinkman, & E. Fendos, 1985. Genotype and media effects on callus formation and regeneration in barley. Crop Sci. 25: 27–31.
Jensen, C. J., 1981. Use of cell and tissue culture techniques in plant breeding and genetics. In: Rachie K. O. & J. M. Lyman (Eds), Genetic engineering for crop improvement. pp. 87–104, Working papers. Rockefeller Foundation, NY.
Lu, C. Y., I. K. Vasil & P. Ozias-Akins, 1982. Somatic embryogenesis in Zea mays L. Theor. Appl. Genet. 62: 109–112.
Lupotto, E., 1984. Callus induction and plant regeneration from barley mature embryos. Ann. Bot. 54: 523–529.
Mix, G., H. M. Wilson & B. Foroughi-Wehr, 1978. The cytological status of plants of Hordeum vulgare L. regenerated from microspore callus. Z. Pflanzenzüchtg 80: 89–99.
Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.
Nabors, M. W., J. W. Heyser, T. A. Dykes & K. J. Demott, 1983. Long duration, high frequency plant regeneration from cereal tissue cultures. Planta 157: 385–391.
Orton, T. J., 1980. Chromosomal variability in tissue cultures and regenerated plants of Hordeum. Theor. Appl. Genet. 56: 101–112.
Scowcroft, W. R., 1984. Genetic variability in tissue cultures: Impact on germplasm conservation and utilization. Tech. Rep. pp. 41. IBPGR Secretariat. Rome.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ahloowalia, B.S. Plant regeneration from embryo-callus culture in barley. Euphytica 36, 659–665 (1987). https://doi.org/10.1007/BF00041516
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00041516