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A gene encoding a major Kunitz proteinase inhibitor of storage organs of winged bean is also expressed in the phloem of stems

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Abstract

Winged bean Kunitz chymotrypsin inhibitor (WCI) accumulates abundantly in seeds and tuberous roots, and small amounts of the WCI protein and mRNA can also be detected in stems. In this study, we analyzed the localization of the WCI protein in stems of winged bean. The results demonstrated that the WCI protein was localized in sieve tubes. Furthermore, we showed that the 5′ region of the WCI-3b gene, which exhibited strong transcriptional activity in developing seeds, also promoted transcription of a reporter gene in the phloem of stems of transgenic tobacco.

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Author notes

  1. Yoshiki Habu

    Present address: National Institute for Basic Biology, 38 Nishigonaka, 444, Modaijicho, Okazaki, Japan

  2. Hisayo Fukushima

    Present address: Scientific Criminal Research Laboratory, Hokkaido Prefectural Police Headquarters, 064, Sapporo, Japan

  3. Yoichi Sakata

    Present address: Department of Agricultural Chemistry, Tokyo University of Agriculture, 156, Sakuragaoka, Japan

  4. Hisashi Abe

    Present address: Forestry and Forest Products Research Institute, Ministry of Agriculture, Forestry and Fisheries, 305, Tsukuba, Japan

Authors and Affiliations

  1. Department of Applied Bioscience, Hokkaido University, 060, Sapporo, Japan

    Yoshiki Habu, Hisayo Fukushima & Yoichi Sakata

  2. Department of Forest Science, Hokkaido University, 060, Sapporo, Japan

    Hisashi Abe & Ryo Funada

Authors
  1. Yoshiki Habu
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  2. Hisayo Fukushima
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  3. Yoichi Sakata
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  4. Hisashi Abe
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  5. Ryo Funada
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Habu, Y., Fukushima, H., Sakata, Y. et al. A gene encoding a major Kunitz proteinase inhibitor of storage organs of winged bean is also expressed in the phloem of stems. Plant Mol Biol 32, 1209–1213 (1996). https://doi.org/10.1007/BF00041408

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  • DOI: https://doi.org/10.1007/BF00041408

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