Abstract
A system of synchronous cell division was established by starvation of auxin and its readdition to suspension cultures of cells of Catharanthus roseus L. cv. Little-Pinky. When cells in the stationary phase were transferred to fresh medium free of 2,4-dichlorophenoxyacetic acid (2,4-D), cells were arrested preferentially at the G1 phase. After cells had been cultured for 2 days in medium without 2,4-D, readdition of 2,4-D induced the synchronous division of cells. In this system, 70–80% of cells divided synchronously within 3 to 4h, and the mitotic index increased sharply in parallel with the increase in cell number. Active synthesis of DNA was demonstrated by measurements of incorporation of [3H]-thymidine into the DNA fraction. The induction of cell division by the addition of 2,4-D was inhibited by treating cells with analogues of auxin, such as 2,4,6-trichlorophenoxyacetic acid and p-chlorophenoxyisobutyric acid.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- DAPI:
-
4,6-diamidino-2-phenylindole
- IAA:
-
indole-3-acetic acid
- MS:
-
Murashige & Skoog
- NAA:
-
α-naphthalenacetic acid
- PCIB:
-
p-chlorophenoxyisobutyric acid
- 2,4,6-T:
-
2,4,6-trichlorophenoxyacetic acid
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Nishida, T., Ohnishi, N., Kodama, H. et al. Establishment of synchrony by starvation and readdition of auxin in suspension cultures of Catharanthus roseus cells. Plant Cell Tiss Organ Cult 28, 37–43 (1992). https://doi.org/10.1007/BF00039913
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DOI: https://doi.org/10.1007/BF00039913