Summary
Hybridizations between three lines of the common bean (Phaseolus vulgaris L.) and two lines of the lima bean (P. lunatus L.) were attempted in order to transfer characters from the lima bean to the common bean. A total of 115 interspecific hybrid embryos were rescued and cultured, and 7 plantlets were eventually transferred to soil. The most compatible cross was ‘Edogawa XPI164891’, which had a high proportion of expanded ovules, and from which we obtained one mature interspecific hybrid. In general, morphological characters of the hybrid were intermediate between the parents, and the chromosome number of the hybrid was 2n=22 the same as that of both parents. The hybrid nature of the progeny plant was confirmed by restriction endonuclease analysis of rDNA. Species specific fragments were detected when total DNA was digested by BamHI, and BamHI-digested total DNA of the hybrid contained all fragments from both parents. Selves and backcrosses were attempted, but no progeny were obtained. The only hybrid obtained was completely sterile and meiosis highly irregular.
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Kuboyama, T., Shintaku, Y. & Takeda, G. Hybrid plant of Phaseolus vulgaris L. and P. lunatus L. obtained by means of embryo rescue and confirmed by restriction endonuclease analysis of rDNA. Euphytica 54, 177–182 (1991). https://doi.org/10.1007/BF00039605
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DOI: https://doi.org/10.1007/BF00039605