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Genetic complementation of a floral homeotic mutation, apetala3, with an Arabidopsis thaliana gene homologous to DEFICIENS of Antirrhinum majus

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Abstract

Among the homeotic mutants with altered floral organs, two mutants of Arabidopsis thaliana, apetala3 and pistillata, and two mutants of Antirrhinum majus, deficiens and globosa, have a homeotic conversion of the floral organs in whorl 2 and 3, namely petals to sepals and stamens to carpels. We have isolated a homologue of the DEFICIENS gene from A. thaliana wild type and shown complete complementation of apetala3 mutation by introducing the isolated gene using Agrobacterium-mediated transformation. These results show that the APETALA3 is a homologue of DEFICIENS structurally and functionally. The 5′-upstream region of APETALA3 contains three SRE-like sequence, where MADS box-containing proteins are assumed to bind and regulate expression in tissue-and stage-specific manner.

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Author notes

  1. Hiroyuki Okamoto

    Present address: Department of Bioapplied Chemistry, Faculty of Technology, Osaka City University, Sumiyoshi-ku, 558, Osaka, Japan

  2. Hideaki Shiraishi

    Present address: Department of Chemistry, Faculty of Science, Kyoto University, 606, Kyoto, Japan

Authors and Affiliations

  1. Division I of Gene Expression and Regulation, National Institute for Basic Biology, 444, Okazaki, Japan

    Hiroyuki Okamoto, Azusa Yano, Hideaki Shiraishi, Kiyotaka Okada & Yoshiro Shimura

  2. Department of Biophysics, Faculty of Science, Kyoto University, 606, Kyoto, Japan

    Yoshiro Shimura

Authors
  1. Hiroyuki Okamoto
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  2. Azusa Yano
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  3. Hideaki Shiraishi
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  4. Kiyotaka Okada
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  5. Yoshiro Shimura
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Okamoto, H., Yano, A., Shiraishi, H. et al. Genetic complementation of a floral homeotic mutation, apetala3, with an Arabidopsis thaliana gene homologous to DEFICIENS of Antirrhinum majus . Plant Mol Biol 26, 465–472 (1994). https://doi.org/10.1007/BF00039556

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  • DOI: https://doi.org/10.1007/BF00039556

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