Abstract
A tissue culture method for regeneration of plantlets from calluses of Dioscorea composita Hemsl. and Dioscorea cayenensis L. is described. Zygotic embryos were used as initial explants. Calluses were obtained on Murashige & Skoog basal medium supplemented with 18 μM 2,4-D and plantlets were regenerated on media containing 0.1 μM zeatin and 3.3 mM glutamine according to previously described protocols [3]. Inclusion of 0.3% (w/v) activated charcoal in media did not increase callusing. Regeneration of plantlets from D. cayenensis calluses occurred only at low levels of 2,4-D (2.25 μM) contained in the media tested. The results indicated that there were genotype-dependent differences between the yam species in their ability to regenerate plantlets in vitro.
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References
Ammirato PV (1978) Somatic embryogenesis and plantlet development in suspension cultures of the medicinal yam, Dioscorea floribunda. Amer J Bot 65 (Suppl):27
Ammirato PV (1982) Growth and morphogenesis in cultures of the monocot yam, Dioscorea. In: Fujiwara A (Ed) Plant Tissue Culture (pp 169–170) Maruzen, Tokyo
Ammirato PV (1984) Yams. In: Ammirato PV, Evans DA, Sharp WR, Yamada Y (Eds) Handbook of Plant Cell Culture, Vol 3, Crop Species (pp 327–354) Macmillan, New York
Ammirato PV (1986) Carrot. In: Evans DA, Sharp WR, Ammirato PV (Eds) Handbook of Plant Cell Culture, Vol 4, Techniques and Applications (pp 457–499) Macmillan, New York
Coursey DG (1967) In: Yams, Longmans, Green and Co, London
Chaturvedi HC (1979) Tissue culture of economic plants. In: Khoshoo TN, Nair PKK (Eds) Progress in Plant Research, Vol. 1, Applied Morphology and Allied Subjects (pp 265–288) Today and Tomorrow's Printers and Publishers, Karolbagh, New Delhi
Funes IJ, Boccon-Gibod J (1988) Culture of yam mesophyll protoplasts (Dioscorea alata, D. bulbifera, D. cayenensis, D. opposita) and obtention of protoplast-divided colonies of (D. alata and D. cayenensis). Can J Bot (in press)
Johansen DA (1940) Plant Microtechnique. McGraw Hill Book Company, New York
Mantell SH, Haque SQ, Whitehall AP (1978) Clonal multiplication of Dioscorea alata L. and Dioscorea rotundata Poir yams by tissue culture. J Hort Sci 53: 95–98
Mantell SH, Haque SQ, Whitehall AP (1980) Apical meristem tip culture for eradication of flexous rod viruses in yams (Dioscorea alata). Trop Pest Management 26: 170–179
Martin FW, Delphin M (1969) Techniques and problems in the propagation of sapogenin-bearing yams from stem cuttings. J Agric Univ Puerto Rico 53: 191–198
Schaefer W (1987) T-DNA expression and integration in a monocotyledonous crop plant. Nature 327: 529–532
Singh JP (1978) Effect of nitrogen on shoot bud differentiation of Dioscorea deltoidea Wall. callus cultures. Biol Plant 20: 436–439
Tisserat B, De Mason DA (1980) A histological study of development of adventitive embryos in organ cultures of Phoenix dactilifera L. Ann Bot 46: 465–472
Vasil V, Vasil IK (1987) Formation of callus and somatic embryos from protoplasts of a commercial hybrid of maize (Zea mays L.) Theor Appl Genet 73: 793–798
Viana AM (1985) Growth and reproduction of Dioscorea composita Hemsl. Ph.D. Thesis, UNICAMP, Campinas, Sao Paulo (Portuguese)
Xinhua F, Quguan S, Xingcun J (1986) Transformation of the monocot Dioscorea opposita using Agrobacterium tumefaciens. Gen Manip in Crops Newsletter 2:52–59
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Viana, A.M., Mantell, S.H. Callus induction and plant regeneration from excised zygotic embryos of the seed-propagated yams Dioscorea composita Hemsl. and D. cayenensis Lam.. Plant Cell Tiss Organ Cult 16, 113–122 (1989). https://doi.org/10.1007/BF00036519
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DOI: https://doi.org/10.1007/BF00036519