Summary
Dry seed lots of a rice cultivar, Harebare, susceptible for bacterial leaf blight (BLB), were exposed to thermal neutrons with and without pre-treatment of seed for boron-enrichment, and to gamma-rays. M1 plants of each of the treatments were grown and their seeds were separately harvested. M2 populations were raised in rice fields of a farmer in a region where BLB is epidemic every year, and M2 and control plants which expressed resistant reactions to BLB were selected. M3 and control lines as plant progenies of the selected M2 plants were raised in the same rice fields as in M2 generation in order to investigate their BLB resistance and other agronomically important traits. Variances for disease severity of M2 populations were significantly larger than those of the control population. whereas their means were not singificantly different from that of the control population, suggesting induction of mutations toward both resistance and susceptibility to BLB. Mean disease severity values of the M3 lines from selected M2 plants were significantly smaller than those of the control lines, indicating gains of selection in M2 for quantitative resistance against BLB. Thermal neutrons, especially with boron-enrichment pre-treatment were effective to induce mutations for resistance against BLB. Some M3 mutant lines with quantitatively enhanced resistance against BLB were not modified in other agronomic traits from those of the original cultivar. Significance of the induced quantitative resistance in breeding programmes for BLB resistance is discussed.
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References
Ando, T., T. Yanamoto & M. Yamada, 1973. Shirahagare byokin ni taisuru inehinsyu no ryoteki teikousei no kenteihoho ni tsuite (in Japanese). Hokuriku-byogaichu-kenkyukaiho. 21: 35–37.
Ezuka, A. & O. Horino, 1974a. Classification of rice varieties and Xanthomonas oryzae strains on the basis of their differential interactions. Bull. Tokai-Kinki Nat. Agr. Exp. Sta. 27: 1–19.
Ezuka, A. & O. Horino, 1974b. Virulence and Aggressiveness in Xanthomonas oryzae. Tokai-Kinki Nat. Agr. Exp. Sta. 27: 26–34.
Ezuka, A. & S. Sakaguchi, 1978. Host-parasite relationship in bacterial leaf blight of rice caused by Xanthomonas oryzae. Rev. Plant protec. Res. 11: 93–118.
Horino, O., 1978. Distributions of pathogenic strains of Xanthomonas oryzae (Uyeda et Ishiyama) Dowson in Japan in 1973 and 1975. Ann. phytopath. Soc. Japan. 44: 297–304.
Horino, O., 1971. A survey of geographical distribution of pathogenic groups of Xanthomonas campestris pv. oryzae from Japan in 1977 and 1979. Ann. Phytopath. Soc. Japan. 47: 50–57.
Horino, O. & T. Yamada, 1980. Inheritance of resistance of IR 26 to bacterial groups I, II, III, IV and V of Xanthomonas campestris pv. oryzae (Ishiyama 1922) Dye 1978 from Japan. Ann. Phytopath. Soc. Japan. 46: 504–509.
Kaku, H., T. Kimura & M. Hori, 1977. Clipping inoculation method for evaluating quantitative resistance of rice varieties to bacterial leaf blight. Chugoku Nat. Agr. Exp. Sta. 54: 17–18.
Kaku, H., T. Kimura & M. Hori, 1980. Evaluation of quantitative resistance of rice cultivars to bacterial leaf blight caused by Xanthomonas oryzae by the clipping inoculation method. Chugoku Nat. Agr. Exp. Sta. 17: 17–32.
Kuhara, S., T. Kurita, Y. Tagami, H. Fujii & H. Sekiya, 1965. Studies on the strain of Xanthomonas oryzae (Uyeda et Ishiyama) Dowson. The pathogen of the bacterial leaf blight of rice, with special reference to its pathogenicity and phage-sensitivity. Kyushu Agr. Exp. Sta. 11: 263–321.
Maruyama, K., F. Kikuchi & M. Yokoo, 1983. Gene analysis of field resistance to rice blast (Pyricularia oryzae) in Rikuto Norin Mochi 4 and its use for breeding. Bull. Nat. Inst. Agr. Sci. D 35: 1–31.
Micke, A., 1974. Scope and aims of the co-ordinated research programme on induced mutations for disease resistance. In. Induced mutations for disease resistance in crop plants. IAEA, Vienna, Pages 3–7.
Morinaka, T., 1978. Ineshirahagarebyo-no-senyosessyu-to-sonoooyo (in Japanese) Syokubutsuboheki 32: 169–173.
Nakai, H. & M. Goto. 1974. An approach for breeding varieties of rice resistant to bacterial leaf blight with induced mutations. J. Agric. Sci. 84: 167–172.
Nakai, H., & M. Goto, 1977. Studies on mutation breeding of rice for bacterial leaf blight resistance. 1. Variations in bacterial leaf blight reactions found in M2 generations. SABRAO Journal 7: 159–170.
Nakai, H. & M. Goto, 1977. Mutation breeding mutations against plant diseases. IAEA, Vienna, Pages 171–186.
Nakai, H. & M. Saito, 1976. The contribution of boron to radiobiological effects of thermal neutrons in rice. Environmental and Experimental Botany. 16: 267–275.
Sakaguchi, S., T. Suwa & N. Murata, 1968. Studies on the resistance to bacterial leaf blight, Xanthomonas oryzae (Uyeda et Ishiyama) Dowson, in the cultivated and wild rice. Bull. Nat. Inst. Agr. Sci. D 18: 1–29.
Shibata, S., S. Okamoto, K. Kanda, T. Kawamoto, K. Araki, K. Sato & T. Kozuka, 1967. Heavy water facility of Kyoto University Reactor. KURRI-TR-28. Res. React. Inst. Kyoto Univ.
Sigurbjörnsson, B. & A. Micke, 1968. Progress in mutation breeding. In: Induced mutations in plants, IAEA, Vienna. Pages 673–698.
Simon, M. D., 1979. Modification of host-parasite interactions through artificial mutagenesis. Ann. Rev. Phytopathol. 17: 75–96.
Washio, O., K. Kariya & K. Toriyama, 1966. Studies on breeding rice varieties for resistance to bacterial leaf blight. Chugoku Nat. Agr. Exp. Sta. A 13: 55–85.
Watanabe, S., 1976. Studies on the breeding of rice varieties resistant to bacterial leaf blight in Sri Lanka. Bull. Tohoku Nat. Agric. Exp. Sta. 54: 1–74.
Yamamoto, T., H. R. Hifni, M. Machmud, T. Nishizawa & D. M. Tantera, 1977. Variation in pathogenicity of Xanthomonas oryzae (Uyeda et Ishiyama) Dowson and resistance of rice varieties to the pathogen. Contr. Centr. Res. Inst. Agric. Bogor. 28: 1–22.
Yamada, T., O. Horino & S. Samoto, 1979a. Studies on genetics and breeding of resistance to bacterial leaf blight in rice. I. Discovery of new varietal groups on the basis of reaction patterns to five different pathotypes of Xanthomonas oryzae (Uyeda et Ishiyama) Dowson in Japan. Ann. Phytopath. Soc. Japan. 45: 239–246.
Yamada, T., O. Horino & S. Samoto, 1979b. Studies on genetics and breeding of resistance to bacterial leaf blight in rice. IV. Inheritance of resistance of IR 28 to bacterial groups I, II, III, IV and V of Xanthomonas oryzae (Uyeda et Ishiyama) Dowson from Japan (English summary on p. 285). Japan. J. Breed. 29: 279–286.
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Nakai, H., Kobayashi, M. & Saito, M. Induction and selection of mutations for resistance against bacterial leaf blight in rice. Euphytica 34, 577–585 (1985). https://doi.org/10.1007/BF00035392
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DOI: https://doi.org/10.1007/BF00035392