Abstract
A full-length cDNA clone (MB3) and three partial clones (MA1, MB1 and MB2) which encode myrosinase (thioglucoside glucohydrolase, EC 3.2.3.1) were isolated from a Sinapis alba (white mustard) cDNA library. Nucleotide sequence analysis of these clones revealed that they are encoded by a gene family. Southern blot analysis with gene-specific probes showed that the gene family consists of a least two subfamilies (MA and MB) each with several members both in S. alba and in Brassica napus (oilseed rape). In Arabidopsis thaliana (wall cress) only three myrosinase genes seem to be present. Northern blot analysis indicated that all the myrosinase mRNA species have the same size, approximately 1.95 kb.
Similar content being viewed by others
References
Bernatzky R, Tanksley SD: Genetics of actin-related sequences in tomato. Theor Appl Genet 72: 314–321 (1986).
Björkman R, Janson J-C: Studies on myrosinase I. Purification and characterization of a myrosinase from white mustard seed (Sinapis alba L.). Biochim Biophys Acta 276: 508–518 (1972).
Bones A, Iversen T-H: Myrosin cells and myrosinase. Isr J Bot 34: 351–375 (1985).
Bones AM, Slupphaug G: Purification, characterization and partial amino acid sequencing of Beta-thioglucosidase from Brassica napus L. J Plant Physiol 134: 722–729 (1989).
Buchwaldt L, Larsen LM, Plöger A, Sörensen HJ: Fast polymer liquid chromatography isolation and characterization of plant myrosinase, β-thioglucoside glucohydrolase isoenzymes. Chromatography 363: 71–80 (1986).
Cleveland DW, Fisher SG, Kirshner MW, Laemmli UK: Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem 252: 1102–1106 (1977).
Fenwick R, Heaney RK, Mullin WJ: Glucosinolates and their breakdown products in food and food plants. CRC Crit Rev Food Sci Nutr 18: 123–201 (1983).
Hawke D, Yuan P: User Bulletin Issue No 28, pp. 1–8. Applied Biosystems (1987).
Höglund A-S, Lenman M, Falk A, Rask L: Distribution of myrosinase in rapeseed tissues. Plant Physiol 95: 213–221 (1991).
Iversen T-H, Baggerud C, Beisvaag T: Myrosin cells in Brassicaceae roots. Z. Pflanzenphysiol 94: 143–154 (1979).
Larsen PO: Glucosinolates. In: Stumpf PK, Tolbert NE, Conn EE (eds) The Biochemistry of Plants, vol 7, pp. 501–525. Academic Press, New York (1980).
Lenman M, Rödin J, Josefsson L-G, Rask L: Immunological characterization of rapeseed myrosinase. Eur J Biochem 194: 747–753 (1990).
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with Folin phenol reagent. J Biol Chem 193: 265–275 (1951).
Lüthy B, Matile P: The mustard oil bomb: Rectified analysis of the subcellular organization of the myrosinase system. Biochem Physiol Pflanzen 179: 5–12 (1984).
Lönnerdal B, Janson J-C: Studies on myrosinase II. Purification and characterization of a myrosinase from rapeseed (Brassica napus L.). Biochim Biophys Acta 315: 421–429 (1973).
Mack DH, Sninsky JJ: A sensitive method for the identification of uncharacterized viruses related to known virus group: Hypadnavirus model system. Proc Natl Acad Sci USA 85: 6977–6981 (1988).
Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).
Mason PJ, Williams JG: Hybridisation in the analysis of recombinant DNA. In: Hames BD, Higgins SJ (eds) Nucleic Acid Hybridization: A Practical Approach, pp. 113–137. IRL Press, Oxford/Washington, DC (1985).
Matsudaira P: Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 261: 10035–10038 (1987).
Selden RF: Analysis of RNA by northern hybridization. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current Protocols in Molecular Biology, pp. 4.9.1–4.9.4. Wiley-Interscience, New York (1987–1989).
Selden RF: Analysis of DNA sequences by blotting and hybridization. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current Protocols in Molecular Biology, pp. 2.9.5–2.9.7. Wiley-Interscience, New York (1987–1989).
Srivastava VK, Hill DC: Glucosinolate hydrolytic products given by Sinapis alba and Brassica napus thioglucosidase. Phytochemistry 13: 1043–1046 (1974).
Thangstad OP, Iversen T-H, Slupphaug G, Bones A: Immunocytochemical localization of myrosinase in Brassica napus. Planta 180: 245–248 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Xue, J., Lenman, M., Falk, A. et al. The glucosinolate-degrading enzyme myrosinase in Brassicaceae is encoded by a gene family. Plant Mol Biol 18, 387–398 (1992). https://doi.org/10.1007/BF00034965
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00034965