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Growth of and quassin accumulation by cultures of Quassia amara

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Abstract

Callus and suspension cultures were established from Quassia amara, a member of the Simaroubaceae. Analysis of the tissue culture showed that quassin was present in both callus and suspension cultures. The effect of variation in auxin and cytokinins on both callus growth and the presence of quassin was examined. The suspension culture was grown in a 7 liter bioreactor when good yields of quassin were achieved.

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Abbreviations

IAA:

indole-3-acetic acid

IBA:

indolebutyric acid

2,4-D:

2,4-dichlorophenoxyacetic acid

NAA:

naphthaleneacetic acid

6BA:

6-benzyladenine

IpA:

N6 (Δ-isopentenyl) adenine

IpAR:

N6 (Δ isopentenyl) adenine riboside

td:

doubling time

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Authors and Affiliations

  1. Wolfson Institute of Biotechnology, Department of Molecular Biology and Biotechnology, University of Sheffield, S10 2TN, Sheffield, UK

    S. Ashton & R. D. Steward

  2. Department of Science, Bristol Polytechnic, Coldharbour Lane, Frenchay, BS16 1Q7, Bristol, UK

    A. H. Scragg

  3. Division of Agriculture, School of Agriculture, University of Aberdeen, 531 King Street, AB9 1UD, Aberdeen, UK

    E. J. Allan

Authors
  1. A. H. Scragg
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  2. S. Ashton
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  3. R. D. Steward
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  4. E. J. Allan
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Scragg, A.H., Ashton, S., Steward, R.D. et al. Growth of and quassin accumulation by cultures of Quassia amara . Plant Cell Tiss Organ Cult 23, 165–169 (1990). https://doi.org/10.1007/BF00034427

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  • DOI: https://doi.org/10.1007/BF00034427

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