Abstract
In vitro micropropagation of Arctostaphylos uva-ursi was performed to increase the number of ground cover species able to serve as substitute for members of the Rosaceae susceptible to fire blight. Explants (node segments) excised from plants growing in the greenhouse were established in vitro on a medium containing 10 μM α-naphthaleneacetic acid (NAA) and activated charcoal (2 g I-1). Using in vitro grown shoots, two propagation procedures were used:
- Culture of nodal fragments with 50 μM NAA resulted in the growth of 6 to 7 nodes every 4 weeks, yielding 1 700 almost rootable shoots after 4 subcultures;
- Development of axillary shoots obtained with media containing 25 μM benzyladenine (BA) and 20 μM indoleacetic acid (IAA) yielded almost 500 rootable shoots after 4 subcultures. The rate of propagation decreased after the 3rd subculture.
Percentage of in vitro rooted shoots reached 98% with diluted micronutrients and 10 μM NAA but 31% of the plants died during acclimatization.
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Abbreviations
- BA:
-
benzyladenine
- BM:
-
basal medium
- HID:
-
high intensity discharge
- IAA:
-
indoleacetic acid
- IBA:
-
indolebutyric acid
- NAA:
-
α-naphthaleneacetic acid
- PAR:
-
photosynthetic active radiation
- 2iP:
-
2-isopentenyladenine
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Kada, J.M., Dorion, N. & Bigot, C. In vitro micropropagation of Arctostaphylos uva-ursi (L.) Sprengel.: Comparison between two methodologies. Plant Cell Tiss Organ Cult 24, 217–222 (1991). https://doi.org/10.1007/BF00033480
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DOI: https://doi.org/10.1007/BF00033480