Abstract
A cell-free translation system was programmed with total, poly(A), non poly(A) and polysomal RNAs from male and female flowers of this plant with separated sexes. The peptide patterns obtained reflected differences in corresponding translatable RNAs. In total RNA products, three peptides were specific for males, two for females. One of the two male-specific polypeptides of high molecular weight was obtained from poly(A) RNAs and a female-specific one from non poly(A) RNAs. Differences between peptides common to both sexes reflected different concentrations of corresponding messengers. Similar results were obtained with polysomal RNAs. The male-specific RNAs were depending on high endogenous auxin concentrations while the female on active cytokinins. Cytokinin feminization of males induced the female-specific RNAs showing cytokinin action at pretranslational stages. Phytohormone roles are discussed.
Similar content being viewed by others
References
Aspart L, Cooke R, Delseny M: Stability of polyadenylic and polyadenylated ribonucleic acids in radish (Raphanus sativus) seedling. Biochem Biophys Acta 564: 43–54, 1979.
Baulcombe DC, Key JL: Polyadenylated RNA sequences which are reduced in concentration following auxin treatment of soybean hypocotyls. J Biol Chem 255: 8907–8913, 1980.
Bazin M, Chabin A, Durand R: Comparison between four isoaccepting transfer ribonucleic acids and corresponding synthetases in male and female flowers of the dioecious species, Mercurialis annua L. Dev Biol 44: 288–297, 1975.
Bostock CJ, Prescott DM, Lauth M: Lability of 26S ribosomal RNA in Tetrahymena pyriformis. Exp Cell Res 66: 260–262, 1971.
Bowden-Bonnets L, Lord JM: Isolation and cell-free translation of total messenger RNA from germinating castor bean endosperm. Plant Physiol 63: 769–773, 1979.
Champault A: Detection et dosage par chromatographie en phase gazeuse de l'acide idolyl-acétique extrait de deux souches tissulaires de l'espèce Mercurialis annua L. (2n=16). C.R. Acad. Sc. Paris, série D280: 591–594, 1975.
Champault A, Chung S, Guerin B, Kahlem G, Lhermitte A, Teller G, Durand R, Durand B: Towards an understanding of the mechanism of cytokinin activity in Mercurialis annua L. sex differentiation. In: Guern J, C Peaud-Lenoël (eds) Metabolism and molecular activities of cytokinins. Springer Verlag Heidelberg, 1981, pp 129–139.
Chantrenne H, Burny A, Marbaix G: The search for the messenger RNA of hemoglobin. Nucleic Acids Res. 7: 173–194, 1976.
Cuming AC, Kennedy TD, Lane BG: Wheat embryo ribonucleates XIV. Mass isolation of mRNA from wheat germ and comparison of its translational capacity with that of mRNA from imbibing wheat embryos. Can J Biochem 57: 1170–1175, 1979.
Dauphin-Guérin B, Teller G, Durand B: Different endogenous cytokinins between male and female Mercurialis annua L. Planta 148: 124–129, 1980.
Dure LIII, Greenway SC, Galau GA: Developmental biochemistry of cottonseed embryogenesis and germination: changing messenger ribonucleic acid populations as shown by in vitro and in vivo protein synthesis. Biochemistry 20: 4162–4168, 1981.
Elliott RM, Davison AN, Lim L: Developmental changes in the protein and ribonucleic acid components of rat brain messenger ribonucleic acid-protein particules isolated from free polysomes by oligo-(dT)-cellulose chromatography. Biochem J 190: 215–223, 1980.
Galau GA, Dure LIII: Developmental biochemistry of cottonseed embryogenesis and germination: changing messenger ribonucleic acid populations as shown by reciprocal heterologous complementary deoxyribonucleic acid-messenger ribonucleic acid hybridization. Biochemistry 20: 4169–4178, 1981.
Gilham PT: The synthesis of polynucleotide-celluloses and their use in the fractionation of polynucleotides. J Chem Am Soc 83: 4982–4985, 1964.
Goldberg RB, Hoschek G, Tam SH, Ditta GS, Breidenbach RW: Abundance, diversity and regulation of mRNA sequence sets in soybean embryogenesis. Dev Biol 83: 201–217, 1981.
Goldstein ES: Translated and sequestral intranslated message sequences in Drosophila oocytes and embryos. Dev Biol 63: 59–66, 1978.
Grierson D, Covey S: Changes in the amount of ribosomal RNA and Poly-(A) containing RNA during leaf development. Planta 127: 77–86, 1976.
Grierson D, Hemleben V: Ribonucleic acid from the higher plant Matthiola incana. Molecular weight measurements and DNA-RNA hybridization studies. Biochem Biophys Acta 475: 424–436, 1977.
Guilfoyle TJ, Lin CY, Chen YM, Nagao RI, Key JL: Enhancement of soybean RNA polymerase I by auxin. Proc Natl Acad Sci USA 72: 69–72, 1975.
Hagenbuchle O, Schibler U, Wiler T: Intermolecular renaturation of hairpin loops leads to stable and specific aggregates of 28 S rRNA and precursors rRNAs from Xenopus laevis. Eur J Biochem 60: 73–82, 1975.
Jackson AO, Larkins BA: Influence of ionic strengh, pH, and chelation of divalent metals on isolation of polyribosomes from tobacco leaves Plant Physiol 57: 5–10, 1976.
Kahlem G, Champault A, Louis JP, Bazin M, Chabin A, Delaigue M, Dauphin B, Durand R, Durand B: Détermination génétique et régulation hormonale de la différenciation sexuelle chez Mercurialis annua L. Physiol Vég 13: 763–779, 1975.
Kahlem G: Isolation and localization by histoimmunology of isoperoxidases specific for male flowers of the dioecious species Mercurialis annua L. Dev Biol 50: 58–67, 1976.
Kamalay JC, Goldberg RB: Regulation of structural gene expression in tobacco. Cell 19: 935–946, 1980.
Key JL, Silflow C: The occurence and distribution of poly(A) ribonucleic acid in Soybean. Plant Physiol 56: 364–369, 1975.
Louis JP, Durand B: Studies with the dioecious Angiosperm Mercurialis annua L. (2n=16). Correlation between genic and cytoplasmic male sterility, sex segregation and feminizing hormones (cytokinins). Mol gen Genet 165: 309–322, 1978.
Lovett JA, Goldstein ES: The cytoplasmic distribution and characterization of Poly(A)-RNA in oocytes and embryos of Drosophila. Dev Biol 61: 70–78, 1977.
Marcus A: Tobacco mosaïc virus ribonucleic acid-dependent aminoacid incorporation in a wheat embryo system in vitro. Analysis of the rate-limiting reactions. J Biol Chem 245: 955–961, 1970.
Melera PW, Davide JP, Hession C: Identification of mRNA in the slime mold Physarum polycephalum. Eur J Biochem 96: 373–378, 1979.
Myhal ML, Hufnagel LA: Poly(A)-RNA from Tetrahymena. Stimulation of protein synthesis in vitro. J. Protozool. 26, 672–675, 1979.
Newbury HJ, Possingham JV: Factors affecting the extraction of intact ribonucleic acid from plant tissues containing interfering phenolic compounds. Plant Physiol 60: 543–547, 1977.
O'Farrell PH: High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250: 4007–4021, 1975.
Pelham HRB, Jackson RJ: An efficient mRNA-dependent translation system from reticulocyte lysates. Eur J Biochem 67: 247–256, 1976.
Pinder JC, Staynov DZ, Gratzer WB: Properties of RNA in formamide. Biochemistry 13: 5367–5373, 1974.
Rosenthal ET, Hunt T, Ruderman JV: Selective translation of mRNA controls of the pattern of protein synthesis during early development of the surf clam, Spinosula solidissima. Cell 20: 487–494, 1980.
Sagher D, Edelman M, Jakob LM: Poly(A) associated RNA in plants. Biochem Biophys Acta 349: 32–38, 1974.
Tessier L, Esnault R: Isolation and characterization of polysomes and polyadenylated polysomal RNA from Vicia faba meristematic root cells. Mol Cell Biochem 29: 173–182, 1980.
Ts'o POP, Helmkamp GK, Sander C: Secondary structure of nucleic acids in organic solvents. II. Optical properties of nucleotides and nucleic acids. Biochem Biophys Acta 55: 584–593, 1962.
Trapy G, Esnault R: Characteristics of Vicia faba meristematic root cells polyadenylated RNAs purified by thermal elution from Poly(U) sepharose. Acides nucléiques et synthèse des protéines chez les végétaux. Coll Int CNRS 261: 381–385, 1977.
Walker JC, Key JL: Isolation of cloned cDNAs to auxin-responsive poly(A)+ RNAs of elongating soybean hypocotyl. Proc Natl Acad Sci USA 79: 7185–7189, 1982.
Wong LJC, Marzluf CA: Sequence complexity and abundance classes of nuclear and polysomal polyadenylated RNA in Neurospora crassa. Biochem Biophys Acta 607: 122–135, 1980.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Delaigue, M., Poulain, T. & Durand, B. Phytohormone control of translatable RNA populations in sexual organogenesis of the dioecious plant Mercurialis annua L. (2n = 16). Plant Mol Biol 3, 419–429 (1984). https://doi.org/10.1007/BF00033390
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00033390