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Activity of the yeast FLP recombinase in Arabidopsis

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Abstract

The coding sequence for FLP recombinase, originally from the 2 μ plasmid of Saccharomyces cerevisiae, was introduced into Arabidopsis behind the cauliflower mosaic virus 35S promoter. FLP activity was monitored by the glucuronidase activity resulting from inversion of an antisense-oriented GUS reporter gene flanked by a pair of FRT target sites in inverted repeat. FLP-dependent Gus activity was observed in both transient assays and transgenic plants. The FLP system will be useful for a variety of in planta genetic manipulations.

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Author notes

  1. Ramesh V. Sonti

    Present address: Center for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India

  2. Alain F. Tissier

    Present address: The Sainsbury Laboratory, John Innès Centre, Colney Lane, NR4 7UH, Norwich, UK

Authors and Affiliations

  1. Department of Biology, Massachusetts Institute of Technology, 02139-4307, Cambridge, MA, USA

    Ramesh V. Sonti, Alain F. Tissier, Donny Wong, Jean-Frédéric Viret & Ethan R. Signer

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  1. Ramesh V. Sonti
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  2. Alain F. Tissier
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  3. Donny Wong
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  4. Jean-Frédéric Viret
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  5. Ethan R. Signer
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Sonti, R.V., Tissier, A.F., Wong, D. et al. Activity of the yeast FLP recombinase in Arabidopsis . Plant Mol Biol 28, 1127–1132 (1995). https://doi.org/10.1007/BF00032673

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  • DOI: https://doi.org/10.1007/BF00032673

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