Abstract
A new cold-regulated (COR) gene, msa CIC, was isolated by differential screening of a cDNA library from cold-acclimated crowns of alfalfa (Medicago sativa L. cv. Apica). Transcripts of msa CIC were not detectable in unacclimated alfalfa and accumulated to higher levels in cold-acclimated plants of the cold-tolerant cv. Apica than in those of the cold-sensitive cv. CUF-101. The DNA sequence analysis of a full-length cDNA clone revealed that msa CIC encodes for a putative protein (MSACIC) of 166 amino acids with distinct proline-rich and hydrophobic domains. Protein sequence comparisons indicated that MSACIC is similar to a group of bimodular proteins that are developmentally regulated in other plant species.
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Castonguay, Y., Laberge, S., Nadeau, P. et al. A cold-induced gene from Medicago sativa encodes a bimodular protein similar to developmentally regulated proteins. Plant Mol Biol 24, 799–804 (1994). https://doi.org/10.1007/BF00029861
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DOI: https://doi.org/10.1007/BF00029861